{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ma L"],"funding":["National High Level Hospital Clinical Research Funding"],"pagination":["8053524"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9883100"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["2023"],"pubmed_abstract":["<h4>Objectives</h4>To establish and evaluate the analytical and clinical performance of the Flash20 SARS-CoV-2 nucleic acid rapid detection system free of RNA extraction.<h4>Methods</h4>The limit of detection (LoD) was determined using a negative nasopharyngeal swab matrix spiked with different concentrations of SARS-CoV-2 virus; a total of 734,337 reference sequences of viral genomes from GenBank were used for the in-silico analysis to assess the inclusivity of the assay. The specificity of the system was evaluated by testing 27 medically relevant organisms. A total of 115 clinical specimens were collected and tested on the Flash20 SARS-CoV-2 detection system and with an FDA-approved comparator test to assess the clinical performance of the system.<h4>Results</h4>The LoD of the Flash20 SA"],"journal":["International journal of analytical chemistry"],"pubmed_title":["Fast and Sensitive Detection of SARS-CoV-2 Nucleic Acid Using a Rapid Detection System Free of RNA Extraction."],"pmcid":["PMC9883100"],"funding_grant_id":["2020","ZRJY2021-GG03","82074221"],"pubmed_authors":["Jiang Y","Liu Y","Zhao M","Gao P","Ma L","Fan Y","Cui Y","Huang H","Cao Y","Kong X"],"additional_accession":[]},"is_claimable":false,"name":"Fast and Sensitive Detection of SARS-CoV-2 Nucleic Acid Using a Rapid Detection System Free of RNA Extraction.","description":"<h4>Objectives</h4>To establish and evaluate the analytical and clinical performance of the Flash20 SARS-CoV-2 nucleic acid rapid detection system free of RNA extraction.<h4>Methods</h4>The limit of detection (LoD) was determined using a negative nasopharyngeal swab matrix spiked with different concentrations of SARS-CoV-2 virus; a total of 734,337 reference sequences of viral genomes from GenBank were used for the in-silico analysis to assess the inclusivity of the assay. The specificity of the system was evaluated by testing 27 medically relevant organisms. A total of 115 clinical specimens were collected and tested on the Flash20 SARS-CoV-2 detection system and with an FDA-approved comparator test to assess the clinical performance of the system.<h4>Results</h4>The LoD of the Flash20 SA","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023","modification":"2026-07-15T22:31:28.167Z","creation":"2025-04-06T14:11:36.339Z"},"accession":"S-EPMC9883100","cross_references":{"pubmed":["36714173"],"doi":["10.1155/2023/8053524"]}}