{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Aguiar VRC"],"funding":["Frederick National Laboratory for Cancer Research","Conselho Nacional de Desenvolvimento Científico e Tecnológico","NIAID NIH HHS","Fundação de Amparo à Pesquisa do Estado de São Paulo","NCI NIH HHS","SANTHE","South African Medical Research Council","National Institute of General Medical Sciences","NIGMS NIH HHS","Division of Intramural Research, National Institute of Allergy and Infectious Diseases"],"pagination":["249-262"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9883133"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["75(3)"],"pubmed_abstract":["Human leukocyte antigen (HLA) class I and II loci are essential elements of innate and acquired immunity. Their functions include antigen presentation to T cells leading to cellular and humoral immune responses, and modulation of NK cells. Their exceptional influence on disease outcome has now been made clear by genome-wide association studies. The exons encoding the peptide-binding groove have been the main focus for determining HLA effects on disease susceptibility/pathogenesis. However, HLA expression levels have also been implicated in disease outcome, adding another dimension to the extreme diversity of HLA that impacts variability in immune responses across individuals. To estimate HLA expression, immunogenetic studies traditionally rely on quantitative PCR (qPCR). Adoption of altern"],"journal":["Immunogenetics"],"pubmed_title":["Comparison between qPCR and RNA-seq reveals challenges of quantifying HLA expression."],"pmcid":["PMC9883133"],"funding_grant_id":["2013/22007-7","HHSN261200800001E","2014/12123-2","2016/24734-1","2012/18010-0","470043/2014-8","GM075091","DEL-15-006","R01 GM075091","AI157850","R01 AI157850"],"pubmed_authors":["Aguiar VRC","Bashirova A","Martin MP","Meyer D","Augusto DG","Ramsuran V","Carrington M","Gutierrez-Arcelus M","Castelli EC","Kulkarni S","Single RM"],"additional_accession":[]},"is_claimable":false,"name":"Comparison between qPCR and RNA-seq reveals challenges of quantifying HLA expression.","description":"Human leukocyte antigen (HLA) class I and II loci are essential elements of innate and acquired immunity. Their functions include antigen presentation to T cells leading to cellular and humoral immune responses, and modulation of NK cells. Their exceptional influence on disease outcome has now been made clear by genome-wide association studies. The exons encoding the peptide-binding groove have been the main focus for determining HLA effects on disease susceptibility/pathogenesis. However, HLA expression levels have also been implicated in disease outcome, adding another dimension to the extreme diversity of HLA that impacts variability in immune responses across individuals. To estimate HLA expression, immunogenetic studies traditionally rely on quantitative PCR (qPCR). Adoption of altern","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jun","modification":"2025-04-04T08:06:22.462Z","creation":"2024-12-04T12:28:14.066Z"},"accession":"S-EPMC9883133","cross_references":{"pubmed":["36707444"],"doi":["10.1007/s00251-023-01296-7"]}}