{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Hsu CG"],"funding":["NIEHS NIH HHS","NHLBI NIH HHS"],"pagination":["131-142"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9886977"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["20(2)"],"pubmed_abstract":["Polyribonucleotide nucleotidyltransferase 1 (Pnpt1) plays critical roles in mitochondrial homeostasis by controlling mitochondrial RNA (mt-RNA) processing, trafficking and degradation. Pnpt1 deficiency results in mitochondrial dysfunction that triggers a type I interferon response, suggesting a role in inflammation. However, the role of Pnpt1 in inflammasome activation remains largely unknown. In this study, we generated myeloid-specific Pnpt1-knockout mice and demonstrated that Pnpt1 depletion enhanced interleukin-1 beta (IL-1β) and interleukin-18 (IL-18) secretion in a mouse sepsis model. Using cultured peritoneal and bone marrow-derived macrophages, we demonstrated that Pnpt1 regulated NLRP3 inflammasome-dependent IL-1β release in response to lipopolysaccharide (LPS), followed by nigeri"],"journal":["Cellular & molecular immunology"],"pubmed_title":["Pnpt1 mediates NLRP3 inflammasome activation by MAVS and metabolic reprogramming in macrophages."],"pmcid":["PMC9886977"],"funding_grant_id":["R01 HL134910","R01 HL140958","P30 ES001247"],"pubmed_authors":["Li W","Chavez CL","Sowden M","Berk BC","Hsu CG"],"additional_accession":[]},"is_claimable":false,"name":"Pnpt1 mediates NLRP3 inflammasome activation by MAVS and metabolic reprogramming in macrophages.","description":"Polyribonucleotide nucleotidyltransferase 1 (Pnpt1) plays critical roles in mitochondrial homeostasis by controlling mitochondrial RNA (mt-RNA) processing, trafficking and degradation. Pnpt1 deficiency results in mitochondrial dysfunction that triggers a type I interferon response, suggesting a role in inflammation. However, the role of Pnpt1 in inflammasome activation remains largely unknown. In this study, we generated myeloid-specific Pnpt1-knockout mice and demonstrated that Pnpt1 depletion enhanced interleukin-1 beta (IL-1β) and interleukin-18 (IL-18) secretion in a mouse sepsis model. Using cultured peritoneal and bone marrow-derived macrophages, we demonstrated that Pnpt1 regulated NLRP3 inflammasome-dependent IL-1β release in response to lipopolysaccharide (LPS), followed by nigeri","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Feb","modification":"2026-07-15T02:53:43.193Z","creation":"2025-04-21T15:56:38.127Z"},"accession":"S-EPMC9886977","cross_references":{"pubmed":["36596874"],"doi":["10.1038/s41423-022-00962-2"]}}