biostudies-literatureGe CChina Postdoctoral Science FoundationNational Natural Science Foundation of ChinaNanjing Science and Technology Development FoundationJiangsu postdoctoral grant program14https://www.ebi.ac.uk/biostudies/studies/S-EPMC9887786biostudies-literatureUnknown23(1)<h4>Background</h4>As a prodrug of 5-fluorouracil (5-FU), orally administrated capecitabine (CAP) undergoes preliminary conversion into active metabolites in the liver and then releases 5-FU in the gut to exert the anti-tumor activity. Since metabolic changes of CAP play a key role in its activation, a single kind of intestinal or hepatic cell can never be used in vitro to evaluate the pharmacokinetics (PK) and pharmacodynamics (PD) nature. Hence, we aimed to establish a novel in vitro system to effectively assess the PK and PD of these kinds of prodrugs.<h4>Methods</h4>Co-culture cellular models were established by simultaneously using colorectal cancer (CRC) and hepatocarcinoma cell lines in one system. Cell Counting Kit-8 (CCK-8) and flow cytometric analysis were used to evaluate cell vCancer cell internationalIn vitro co-culture systems of hepatic and intestinal cells for cellular pharmacokinetic and pharmacodynamic studies of capecitabine against colorectal cancer.PMC98877862018Z09281803624202205029818036222018M640498Ge CZou JZhang JZhu YXu CFeng DYuan MZhang SJie QAa JTan ZZhang YHuang XfalseIn vitro co-culture systems of hepatic and intestinal cells for cellular pharmacokinetic and pharmacodynamic studies of capecitabine against colorectal cancer.<h4>Background</h4>As a prodrug of 5-fluorouracil (5-FU), orally administrated capecitabine (CAP) undergoes preliminary conversion into active metabolites in the liver and then releases 5-FU in the gut to exert the anti-tumor activity. Since metabolic changes of CAP play a key role in its activation, a single kind of intestinal or hepatic cell can never be used in vitro to evaluate the pharmacokinetics (PK) and pharmacodynamics (PD) nature. Hence, we aimed to establish a novel in vitro system to effectively assess the PK and PD of these kinds of prodrugs.<h4>Methods</h4>Co-culture cellular models were established by simultaneously using colorectal cancer (CRC) and hepatocarcinoma cell lines in one system. Cell Counting Kit-8 (CCK-8) and flow cytometric analysis were used to evaluate cell v2023-01-01T00:00:00Z2023 Jan2025-04-04T09:19:20.864Z2025-04-04T09:19:20.864ZS-EPMC98877863671784510.1186/s12935-023-02853-6