{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yu R"],"funding":["National Natural Science Foundation of China"],"pagination":["1333-1347"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9889532"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["50(2)"],"pubmed_abstract":["Background
The microenvironment of hypoxia is an important factor contributing to the development of glioblastoma (GBM). MicroRNA-588 and its potential target Roundabout-directed receptor 1 (ROBO1) have been reported to promote tumor invasion and proliferation in diseases such as gastric, pancreatic and hepatocellular carcinoma, while their function in GBM and response to hypoxic states remain elusive.Methods
A microarray was leveraged to identify differentially expressed microRNAs in U251 glioma cells cultured under normoxic and hypoxic conditions. The expression of miR-588 was assessed using quantitative real-time PCR (qRT‒PCR). Gain- and loss-of-function studies were used to evaluate the role of miR-588 under hypoxic and normoxic conditions. Cell invasion, migration, pro"],"journal":["Molecular biology reports"],"pubmed_title":["MicroRNA-588 regulates the invasive, migratory and vasculogenic mimicry-forming abilities of hypoxic glioma cells by targeting ROBO1."],"pmcid":["PMC9889532"],"funding_grant_id":["81902540；81874082","82072775","82072776","81802966","81472353","81874083","81702468"],"pubmed_authors":["Li G","Yu R","Zhao R","Zhang Z","Wang S","Gao X","Sun X","Sun Z","Xue H"],"additional_accession":[]},"is_claimable":false,"name":"MicroRNA-588 regulates the invasive, migratory and vasculogenic mimicry-forming abilities of hypoxic glioma cells by targeting ROBO1.","description":"Background
The microenvironment of hypoxia is an important factor contributing to the development of glioblastoma (GBM). MicroRNA-588 and its potential target Roundabout-directed receptor 1 (ROBO1) have been reported to promote tumor invasion and proliferation in diseases such as gastric, pancreatic and hepatocellular carcinoma, while their function in GBM and response to hypoxic states remain elusive.Methods
A microarray was leveraged to identify differentially expressed microRNAs in U251 glioma cells cultured under normoxic and hypoxic conditions. The expression of miR-588 was assessed using quantitative real-time PCR (qRT‒PCR). Gain- and loss-of-function studies were used to evaluate the role of miR-588 under hypoxic and normoxic conditions. Cell invasion, migration, pro","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Feb","modification":"2025-04-21T22:48:38.406Z","creation":"2025-04-05T18:52:21.557Z"},"accession":"S-EPMC9889532","cross_references":{"pubmed":["36459288"],"doi":["10.1007/s11033-022-08063-z"]}}