{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Xiao Y"],"funding":["Chinese Academy of Sciences","Zhejiang Provincial Postdoctoral Science Foundation","National Natural Science Foundation of China","National Key Research and Development Program of China"],"pagination":["e111364"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9890236"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["42(3)"],"pubmed_abstract":["Early embryonic development depends on proper utilization and clearance of maternal transcriptomes. How these processes are spatiotemporally regulated remains unclear. Here we show that nuclear RNA-binding protein Rbm14 and maternal mRNAs co-phase separate into cytoplasmic condensates to facilitate vertebrate blastula-to-gastrula development. In zebrafish, Rbm14 condensates were highly abundant in blastomeres and markedly reduced after prominent activation of zygotic transcription. They concentrated at spindle poles by associating with centrosomal γ-tubulin puncta and displayed mainly asymmetric divisions with a global symmetry across embryonic midline in 8- and 16-cell embryos. Their formation was dose-dependently stimulated by m<sup>6</sup> A, but repressed by m<sup>5</sup> C modificatio"],"journal":["The EMBO journal"],"pubmed_title":["Maternal mRNA deadenylation and allocation via Rbm14 condensates facilitate vertebrate blastula development."],"pmcid":["PMC9890236"],"funding_grant_id":["32000556","XDB19020102","2017YFA0503500","E1BH3B0401"],"pubmed_authors":["Li J","Chen J","Xiao Y","Jing N","Xie L","Zhu X","Sun H","Yang S"],"additional_accession":[]},"is_claimable":false,"name":"Maternal mRNA deadenylation and allocation via Rbm14 condensates facilitate vertebrate blastula development.","description":"Early embryonic development depends on proper utilization and clearance of maternal transcriptomes. How these processes are spatiotemporally regulated remains unclear. Here we show that nuclear RNA-binding protein Rbm14 and maternal mRNAs co-phase separate into cytoplasmic condensates to facilitate vertebrate blastula-to-gastrula development. In zebrafish, Rbm14 condensates were highly abundant in blastomeres and markedly reduced after prominent activation of zygotic transcription. They concentrated at spindle poles by associating with centrosomal γ-tubulin puncta and displayed mainly asymmetric divisions with a global symmetry across embryonic midline in 8- and 16-cell embryos. Their formation was dose-dependently stimulated by m<sup>6</sup> A, but repressed by m<sup>5</sup> C modificatio","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Feb","modification":"2026-07-14T21:53:16.239Z","creation":"2024-10-15T10:39:13.459Z"},"accession":"S-EPMC9890236","cross_references":{"pubmed":["36477743"],"doi":["10.15252/embj.2022111364"]}}