biostudies-literatureXiao YChinese Academy of SciencesZhejiang Provincial Postdoctoral Science FoundationNational Natural Science Foundation of ChinaNational Key Research and Development Program of Chinae111364https://www.ebi.ac.uk/biostudies/studies/S-EPMC9890236biostudies-literatureUnknown42(3)Early embryonic development depends on proper utilization and clearance of maternal transcriptomes. How these processes are spatiotemporally regulated remains unclear. Here we show that nuclear RNA-binding protein Rbm14 and maternal mRNAs co-phase separate into cytoplasmic condensates to facilitate vertebrate blastula-to-gastrula development. In zebrafish, Rbm14 condensates were highly abundant in blastomeres and markedly reduced after prominent activation of zygotic transcription. They concentrated at spindle poles by associating with centrosomal γ-tubulin puncta and displayed mainly asymmetric divisions with a global symmetry across embryonic midline in 8- and 16-cell embryos. Their formation was dose-dependently stimulated by m⁶ A, but repressed by m⁵ C modificatioThe EMBO journalMaternal mRNA deadenylation and allocation via Rbm14 condensates facilitate vertebrate blastula development.PMC989023632000556XDB190201022017YFA0503500E1BH3B0401Li JChen JXiao YJing NXie LZhu XSun HYang SfalseMaternal mRNA deadenylation and allocation via Rbm14 condensates facilitate vertebrate blastula development.Early embryonic development depends on proper utilization and clearance of maternal transcriptomes. How these processes are spatiotemporally regulated remains unclear. Here we show that nuclear RNA-binding protein Rbm14 and maternal mRNAs co-phase separate into cytoplasmic condensates to facilitate vertebrate blastula-to-gastrula development. In zebrafish, Rbm14 condensates were highly abundant in blastomeres and markedly reduced after prominent activation of zygotic transcription. They concentrated at spindle poles by associating with centrosomal γ-tubulin puncta and displayed mainly asymmetric divisions with a global symmetry across embryonic midline in 8- and 16-cell embryos. Their formation was dose-dependently stimulated by m⁶ A, but repressed by m⁵ C modificatio2023-01-01T00:00:00Z2023 Feb2026-07-14T21:53:16.239Z2024-10-15T10:39:13.459ZS-EPMC98902363647774310.15252/embj.2022111364