biostudies-literatureLuo LAmerican Heart AssociationNHLBI NIH HHSNational Institute of Health2703-2717https://www.ebi.ac.uk/biostudies/studies/S-EPMC9890476biostudies-literatureUnknown118(12)<h4>Aims</h4>Intimal hyperplasia is a common feature of vascular remodelling disorders. Accumulation of synthetic smooth muscle cell (SMC)-like cells is the main underlying cause. Current therapeutic approaches including drug-eluting stents are not perfect due to the toxicity on endothelial cells and novel therapeutic strategies are needed. Our preliminary screening for dysregulated cyclic nucleotide phosphodiesterases (PDEs) in growing SMCs revealed the alteration of PDE10A expression. Herein, we investigated the function of PDE10A in SMC proliferation and intimal hyperplasia both in vitro and in vivo.<h4>Methods and results</h4>RT-qPCR, immunoblot, and in situ proximity ligation assay were performed to determine PDE10A expression in synthetic SMCs and injured vessels. We found that PDE10Cardiovascular researchRole of PDE10A in vascular smooth muscle cell hyperplasia and pathological vascular remodelling.PMC9890476R01 HL13491018PRE34030228R01 HL139794R01 HL154318R01 HL122686R01HL134910R01HL154318Long XZhang YLuo LCai YKorshunov VABerk BCKnight PAYan CHsu CGfalseRole of PDE10A in vascular smooth muscle cell hyperplasia and pathological vascular remodelling.<h4>Aims</h4>Intimal hyperplasia is a common feature of vascular remodelling disorders. Accumulation of synthetic smooth muscle cell (SMC)-like cells is the main underlying cause. Current therapeutic approaches including drug-eluting stents are not perfect due to the toxicity on endothelial cells and novel therapeutic strategies are needed. Our preliminary screening for dysregulated cyclic nucleotide phosphodiesterases (PDEs) in growing SMCs revealed the alteration of PDE10A expression. Herein, we investigated the function of PDE10A in SMC proliferation and intimal hyperplasia both in vitro and in vivo.<h4>Methods and results</h4>RT-qPCR, immunoblot, and in situ proximity ligation assay were performed to determine PDE10A expression in synthetic SMCs and injured vessels. We found that PDE102022-01-01T00:00:00Z2022 Sep2026-06-01T03:16:59.401Z2025-02-18T23:37:24.126ZS-EPMC98904763455032210.1093/cvr/cvab304