{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Birkholz EA"],"funding":["NIAID NIH HHS","NIGMS NIH HHS"],"pagination":["111179"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9891218"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["40(7)"],"pubmed_abstract":["Nucleus-forming jumbo phages establish an intricate subcellular organization, enclosing phage genomes within a proteinaceous shell called the phage nucleus. During infection in Pseudomonas, some jumbo phages assemble a bipolar spindle of tubulin-like PhuZ filaments that positions the phage nucleus at midcell and drives its intracellular rotation. This facilitates the distribution of capsids on its surface for genome packaging. Here we show that the Escherichia coli jumbo phage Goslar assembles a phage nucleus surrounded by an array of PhuZ filaments resembling a vortex instead of a bipolar spindle. Expression of a mutant PhuZ protein strongly reduces Goslar phage nucleus rotation, demonstrating that the PhuZ cytoskeletal vortex is necessary for rotating the phage nucleus. While vortex-like"],"journal":["Cell reports"],"pubmed_title":["A cytoskeletal vortex drives phage nucleus rotation during jumbo phage replication in E. coli."],"pmcid":["PMC9891218"],"funding_grant_id":["R21 AI148814","R01 GM129245"],"pubmed_authors":["Armbruster E","Laughlin TG","Pogliano J","Lee J","Villa E","Suslov S","Wittmann J","Corbett KD","Birkholz EA"],"additional_accession":[]},"is_claimable":false,"name":"A cytoskeletal vortex drives phage nucleus rotation during jumbo phage replication in E. coli.","description":"Nucleus-forming jumbo phages establish an intricate subcellular organization, enclosing phage genomes within a proteinaceous shell called the phage nucleus. During infection in Pseudomonas, some jumbo phages assemble a bipolar spindle of tubulin-like PhuZ filaments that positions the phage nucleus at midcell and drives its intracellular rotation. This facilitates the distribution of capsids on its surface for genome packaging. Here we show that the Escherichia coli jumbo phage Goslar assembles a phage nucleus surrounded by an array of PhuZ filaments resembling a vortex instead of a bipolar spindle. Expression of a mutant PhuZ protein strongly reduces Goslar phage nucleus rotation, demonstrating that the PhuZ cytoskeletal vortex is necessary for rotating the phage nucleus. While vortex-like","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Aug","modification":"2025-04-21T15:10:32.631Z","creation":"2025-04-21T15:10:32.631Z"},"accession":"S-EPMC9891218","cross_references":{"pubmed":["35977483"],"doi":["10.1016/j.celrep.2022.111179"]}}