{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Claes BSR"],"funding":["U.S. Department of Health and Human Services","Dutch Province of Limburg"],"pagination":["2329-2338"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9893213"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["95(4)"],"pubmed_abstract":["Recently, a novel technology was published, utilizing the strengths of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) and immunohistochemistry (IHC), achieving highly multiplexed, targeted imaging of biomolecules in tissue. This new technique, called MALDI-IHC, opened up workflows to target molecules of interest using MALDI-MSI that are usually targeted by standard IHC. In this paper, the utility of targeted MALDI-IHC and its complementarity with untargeted on-tissue bottom-up spatial proteomics is explored using breast cancer tissue. Furthermore, the MALDI-2 effect was investigated and demonstrated to improve MALDI-IHC. Formalin-fixed paraffin-embedded (FFPE) human breast cancer tissue sections were stained for multiplex MALDI-IHC with six photocleavab"],"journal":["Analytical chemistry"],"pubmed_title":["MALDI-IHC-Guided In-Depth Spatial Proteomics: Targeted and Untargeted MSI Combined."],"pmcid":["PMC9893213"],"funding_grant_id":["R01 CA213492","CA236097"],"pubmed_authors":["Krestensen KK","Kuik C","Claes BSR","Heeren RMA","Lim MJ","Vandenbosch M","Grgic A","Rothschild KJ","Yagnik G"],"additional_accession":[]},"is_claimable":false,"name":"MALDI-IHC-Guided In-Depth Spatial Proteomics: Targeted and Untargeted MSI Combined.","description":"Recently, a novel technology was published, utilizing the strengths of matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) and immunohistochemistry (IHC), achieving highly multiplexed, targeted imaging of biomolecules in tissue. This new technique, called MALDI-IHC, opened up workflows to target molecules of interest using MALDI-MSI that are usually targeted by standard IHC. In this paper, the utility of targeted MALDI-IHC and its complementarity with untargeted on-tissue bottom-up spatial proteomics is explored using breast cancer tissue. Furthermore, the MALDI-2 effect was investigated and demonstrated to improve MALDI-IHC. Formalin-fixed paraffin-embedded (FFPE) human breast cancer tissue sections were stained for multiplex MALDI-IHC with six photocleavab","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jan","modification":"2025-04-26T18:10:39.462Z","creation":"2025-04-06T15:43:12.582Z"},"accession":"S-EPMC9893213","cross_references":{"pubmed":["36638208"],"doi":["10.1021/acs.analchem.2c04220"]}}