{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Deng M"],"funding":["Natural Science Foundation of Hubei Province","Hubei Provincial Department of Science & Technology","Tongji Hospital Returnees Initiation Fund","Overseas Expertise Introduction Center for Discipline Innovation","Jianghan University","National Natural Science Foundation of China","Opening Project of Key Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of Education"],"pagination":["e2205886"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9896035"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["10(4)"],"pubmed_abstract":["The incidence of prostate cancer (PCa) in men globally increases as the standard of living improves. Blood serum biomarker prostate-specific antigen (PSA) detection is the gold standard assay that do not meet the requirements of early detection. Herein, a solution-gated graphene transistor (SGGT) biosensor for the ultrasensitive and rapid quantification detection of the early prostate cancer-relevant biomarker, miRNA-21 is reported. The designed single-stranded DNA (ssDNA) probes immobilized on the Au gate can hybridize effectively with the miRNA-21 molecules targets and induce the Dirac voltage shifts of SGGT transfer curves. The limit of detection (LOD) of the sensor can reach 10<sup>-20</sup>  M without amplification and any chemical or biological labeling. The detection linear range is from 10<sup>-20</sup> to 10<sup>-12</sup>  M. The sensor can realize real-time detection of the miRNA-21 molecules in less than 5 min and can well distinguish one-mismatched miRNA-21 molecule. The blood serum samples from the patients without RNA extraction and amplification are measured. The results demonstrated that the biosensor can well distinguish the cancer patients from the control group and has higher sensitivity (100%) than PSA detection (58.3%). Contrastingly, it can be found that the PSA level is not directly related to PCa."],"journal":["Advanced science (Weinheim, Baden-Wurttemberg, Germany)"],"pubmed_title":["Unamplified and Real-Time Label-Free miRNA-21 Detection Using Solution-Gated Graphene Transistors in Prostate Cancer Diagnosis."],"pmcid":["PMC9896035"],"funding_grant_id":["51673060","2018CKB910","JDGD-202205","2020HGRY016","2020BIB020","JDGD‐202205","D18025"],"pubmed_authors":["Yang H","Li Z","Zhang D","Li J","Xue C","Ren Z","Zhang H","Deng M","Wang X","Wang J"],"additional_accession":[]},"is_claimable":false,"name":"Unamplified and Real-Time Label-Free miRNA-21 Detection Using Solution-Gated Graphene Transistors in Prostate Cancer Diagnosis.","description":"The incidence of prostate cancer (PCa) in men globally increases as the standard of living improves. Blood serum biomarker prostate-specific antigen (PSA) detection is the gold standard assay that do not meet the requirements of early detection. Herein, a solution-gated graphene transistor (SGGT) biosensor for the ultrasensitive and rapid quantification detection of the early prostate cancer-relevant biomarker, miRNA-21 is reported. The designed single-stranded DNA (ssDNA) probes immobilized on the Au gate can hybridize effectively with the miRNA-21 molecules targets and induce the Dirac voltage shifts of SGGT transfer curves. The limit of detection (LOD) of the sensor can reach 10<sup>-20</sup>  M without amplification and any chemical or biological labeling. The detection linear range is from 10<sup>-20</sup> to 10<sup>-12</sup>  M. The sensor can realize real-time detection of the miRNA-21 molecules in less than 5 min and can well distinguish one-mismatched miRNA-21 molecule. The blood serum samples from the patients without RNA extraction and amplification are measured. The results demonstrated that the biosensor can well distinguish the cancer patients from the control group and has higher sensitivity (100%) than PSA detection (58.3%). Contrastingly, it can be found that the PSA level is not directly related to PCa.","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Feb","modification":"2025-04-21T18:14:42.721Z","creation":"2025-04-05T17:07:37.778Z"},"accession":"S-EPMC9896035","cross_references":{"pubmed":["36480308"],"doi":["10.1002/advs.202205886"]}}