biostudies-literatureZhang XScientific Research of Department of Science and Technology of Liaoning Province108-120https://www.ebi.ac.uk/biostudies/studies/S-EPMC9896159biostudies-literatureUnknown9(2)The classification of multifocal lung adenocarcinomas (MLAs), including multiple primary lung adenocarcinomas (MPLAs) and intrapulmonary metastases (IPMs), has great clinical significance in staging and treatment determination. However, the application of molecular approaches in pN0M0 MLA diagnosis has not been well investigated. Here, we performed next-generation sequencing (NGS) analysis in 45 pN0M0 MLA patients (101 lesion pairs) who were initially diagnosed as having MPLA by comprehensive histologic assessment (CHA). Five additional patients with intrathoracic metastases were used as positive controls, while 197 patients with unifocal lung adenocarcinomas (425 random lesion pairs) were used as negative controls. By utilizing a predefined NGS criterion, all IPMs in the positive control The journal of pathology. Clinical researchA novel NGS-based diagnostic algorithm for classifying multifocal lung adenocarcinomas in pN0M0 patients.PMC98961592017225035Ren XFan XZhang XSun CYang PMiao YXu SWang LXu YWu XfalseA novel NGS-based diagnostic algorithm for classifying multifocal lung adenocarcinomas in pN0M0 patients.The classification of multifocal lung adenocarcinomas (MLAs), including multiple primary lung adenocarcinomas (MPLAs) and intrapulmonary metastases (IPMs), has great clinical significance in staging and treatment determination. However, the application of molecular approaches in pN0M0 MLA diagnosis has not been well investigated. Here, we performed next-generation sequencing (NGS) analysis in 45 pN0M0 MLA patients (101 lesion pairs) who were initially diagnosed as having MPLA by comprehensive histologic assessment (CHA). Five additional patients with intrathoracic metastases were used as positive controls, while 197 patients with unifocal lung adenocarcinomas (425 random lesion pairs) were used as negative controls. By utilizing a predefined NGS criterion, all IPMs in the positive control 2023-01-01T00:00:00Z2023 Mar2025-04-18T23:25:22.164Z2025-04-07T10:59:17.977ZS-EPMC98961593657955010.1002/cjp2.306