{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Guo W"],"funding":["Science and Technology Commission of Shanghai Municipality"],"pagination":["81-94"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9898265"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["55(1)"],"pubmed_abstract":["The identification of predictive markers to determine the triggering phase prior to the onset of osteoporosis is essential to mitigate further irrevocable deterioration. To determine the early warning signs before osteoporosis, we used the dynamic network biomarker (DNB) approach to analyze time-series gene expression data in a zebrafish osteoporosis model, which revealed that cyclin-dependent kinase inhibitor 1 A (cdkn1a) is a core DNB. We found that cdkn1a negatively regulates osteogenesis, as evidenced by loss-of-function and gain-of-function studies. Specifically, CRISPR/Cas9-mediated cdkn1a knockout in zebrafish significantly altered skeletal development and increased bone mineralization, whereas inducible cdkn1a expression significantly contributed to osteoclast differentiation. We a"],"journal":["Experimental & molecular medicine"],"pubmed_title":["Dynamic network biomarker identifies cdkn1a-mediated bone mineralization in the triggering phase of osteoporosis."],"pmcid":["PMC9898265"],"funding_grant_id":["19441906200"],"pubmed_authors":["Li H","Liu Z","Xia L","Guo W","Zhou T","Jin P","Li R","Huang L","Fang B"],"additional_accession":[]},"is_claimable":false,"name":"Dynamic network biomarker identifies cdkn1a-mediated bone mineralization in the triggering phase of osteoporosis.","description":"The identification of predictive markers to determine the triggering phase prior to the onset of osteoporosis is essential to mitigate further irrevocable deterioration. To determine the early warning signs before osteoporosis, we used the dynamic network biomarker (DNB) approach to analyze time-series gene expression data in a zebrafish osteoporosis model, which revealed that cyclin-dependent kinase inhibitor 1 A (cdkn1a) is a core DNB. We found that cdkn1a negatively regulates osteogenesis, as evidenced by loss-of-function and gain-of-function studies. Specifically, CRISPR/Cas9-mediated cdkn1a knockout in zebrafish significantly altered skeletal development and increased bone mineralization, whereas inducible cdkn1a expression significantly contributed to osteoclast differentiation. We a","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jan","modification":"2026-05-28T23:59:16.613Z","creation":"2024-12-03T22:58:12.422Z"},"accession":"S-EPMC9898265","cross_references":{"pubmed":["36599933"],"doi":["10.1038/s12276-022-00915-9"]}}