biostudies-literatureWang WNational Natural Science Foundation of China82https://www.ebi.ac.uk/biostudies/studies/S-EPMC9899399biostudies-literatureUnknown21(1)<h4>Background</h4>Our previous study showed that fucosyltransferase 2 (Fut2) deficiency is closely related to colitis. Colitis increases the risk for the development of colorectal cancer (CRC). This study aimed to investigate the effect and underlying mechanism of action of Fut2 in CRC.<h4>Methods</h4>Intestinal epithelium-specific Fut2 knockout (Fut2^△IEC) mice were used in this study. CRC was induced using azoxymethane (AOM) and dextran sulfate sodium (DSS). Immunofluorescence was used to examine the fucosylation levels. Proteomics and N-glycoproteomics analyses, Ulex Europaeus Agglutinin I (UEA-I) affinity chromatography, immunoprecipitation, and rescue assay were used to investigate the mechanism of Fut2 in CRC.<h4>Results</h4>The expression of Fut2 and α-1,2-fucosylation waJournal of translational medicineIntestinal epithelium-specific Fut2 deficiency promotes colorectal cancer through down-regulating fucosylation of MCAM.PMC98993998197406882203338821705718227058681900580Qian WLin RJiang XDuan CTian SWang WHan CHou XTang XfalseIntestinal epithelium-specific Fut2 deficiency promotes colorectal cancer through down-regulating fucosylation of MCAM.<h4>Background</h4>Our previous study showed that fucosyltransferase 2 (Fut2) deficiency is closely related to colitis. Colitis increases the risk for the development of colorectal cancer (CRC). This study aimed to investigate the effect and underlying mechanism of action of Fut2 in CRC.<h4>Methods</h4>Intestinal epithelium-specific Fut2 knockout (Fut2^△IEC) mice were used in this study. CRC was induced using azoxymethane (AOM) and dextran sulfate sodium (DSS). Immunofluorescence was used to examine the fucosylation levels. Proteomics and N-glycoproteomics analyses, Ulex Europaeus Agglutinin I (UEA-I) affinity chromatography, immunoprecipitation, and rescue assay were used to investigate the mechanism of Fut2 in CRC.<h4>Results</h4>The expression of Fut2 and α-1,2-fucosylation wa2023-01-01T00:00:00Z2023 Feb2026-07-14T21:14:43.907Z2024-11-13T06:05:17.87ZS-EPMC98993993673942810.1186/s12967-023-03906-0