{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kundu S"],"funding":["Foundation for the National Institutes of Health","National Institutes of Health","National Institute of General Medical Sciences","NIGMS NIH HHS"],"pagination":["103-109"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9899547"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["645"],"pubmed_abstract":["Glycosylphosphatidylinositol (GPI) anchorage is one of the most common mechanisms to attach proteins to the plasma membrane of eukaryotic cells. GPI-anchored proteins (GPI-APs) play a critical role in many biological processes but are difficult to study. Here, a new method was developed for the effective and selective metabolic engineering and labeling of cell surface GPI-APs with an azide-modified phosphatidylinositol (PI) as the biosynthetic precursor of GPIs. It was demonstrated that this azido-PI derivative was taken up by HeLa cells and incorporated into the biosynthetic pathway of GPIs to present azide-labeled GPI-APs on the live cell surface. The azido group was used as a molecular handle to install other labels through a biocompatible click reaction to enable various biological stu"],"journal":["Biochemical and biophysical research communications"],"pubmed_title":["Labeling cell surface glycosylphosphatidylinositol-anchored proteins through metabolic engineering using an azide-modified phosphatidylinositol."],"pmcid":["PMC9899547"],"funding_grant_id":["R35 GM131686","1R35 GM131686"],"pubmed_authors":["Guo J","Guo Z","Craig KC","Jaiswal M","Kundu S"],"additional_accession":[]},"is_claimable":false,"name":"Labeling cell surface glycosylphosphatidylinositol-anchored proteins through metabolic engineering using an azide-modified phosphatidylinositol.","description":"Glycosylphosphatidylinositol (GPI) anchorage is one of the most common mechanisms to attach proteins to the plasma membrane of eukaryotic cells. GPI-anchored proteins (GPI-APs) play a critical role in many biological processes but are difficult to study. Here, a new method was developed for the effective and selective metabolic engineering and labeling of cell surface GPI-APs with an azide-modified phosphatidylinositol (PI) as the biosynthetic precursor of GPIs. It was demonstrated that this azido-PI derivative was taken up by HeLa cells and incorporated into the biosynthetic pathway of GPIs to present azide-labeled GPI-APs on the live cell surface. The azido group was used as a molecular handle to install other labels through a biocompatible click reaction to enable various biological stu","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Feb","modification":"2025-04-22T09:53:57.076Z","creation":"2025-04-05T23:17:33.183Z"},"accession":"S-EPMC9899547","cross_references":{"pubmed":["36682329"],"doi":["10.1016/j.bbrc.2023.01.029"]}}