{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Pons S"],"funding":["NIAID NIH HHS"],"pagination":["104439"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9900374"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["88"],"pubmed_abstract":["<h4>Background</h4>Worldwide, Escherichia coli is the leading cause of neonatal Gram-negative bacterial meningitis, but full understanding of the pathogenesis of this disease is not yet achieved. Moreover, to date, no vaccine is available against bacterial neonatal meningitis.<h4>Methods</h4>Here, we used Transposon Sequencing of saturated banks of mutants (TnSeq) to evaluate E. coli K1 genetic fitness in murine neonatal meningitis. We identified E. coli K1 genes encoding for factors important for systemic dissemination and brain infection, and focused on products with a likely outer-membrane or extra-cellular localization, as these are potential vaccine candidates. We used in vitro and in vivo models to study the efficacy of active and passive immunization.<h4>Results</h4>We selected for "],"journal":["EBioMedicine"],"pubmed_title":["A high-throughput sequencing approach identifies immunotherapeutic targets for bacterial meningitis in neonates."],"pmcid":["PMC9900374"],"funding_grant_id":["R01 AI046706","R01 AI026289"],"pubmed_authors":["Pons S","Roux D","Kropec A","Danilchanka O","Lebreton F","Angoulvant F","Frapy E","Aschard H","Skurnik D","Allain M","Lecuyer H","Sokol H","Guillard T","Gaultier C","Sereme Y","Schlemmer L","Pier GB","Bonacorsi S","Mekalanos JJ","Cywes-Bentley C","Schrimpf C"],"additional_accession":[]},"is_claimable":false,"name":"A high-throughput sequencing approach identifies immunotherapeutic targets for bacterial meningitis in neonates.","description":"<h4>Background</h4>Worldwide, Escherichia coli is the leading cause of neonatal Gram-negative bacterial meningitis, but full understanding of the pathogenesis of this disease is not yet achieved. Moreover, to date, no vaccine is available against bacterial neonatal meningitis.<h4>Methods</h4>Here, we used Transposon Sequencing of saturated banks of mutants (TnSeq) to evaluate E. coli K1 genetic fitness in murine neonatal meningitis. We identified E. coli K1 genes encoding for factors important for systemic dissemination and brain infection, and focused on products with a likely outer-membrane or extra-cellular localization, as these are potential vaccine candidates. We used in vitro and in vivo models to study the efficacy of active and passive immunization.<h4>Results</h4>We selected for ","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Feb","modification":"2026-03-18T13:48:22.553Z","creation":"2025-04-04T08:31:12.583Z"},"accession":"S-EPMC9900374","cross_references":{"pubmed":["36709579"],"doi":["10.1016/j.ebiom.2023.104439"]}}