{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Ebrahimi V"],"funding":["European Research Council","NIDDK NIH HHS","Medical Research Council","Biotechnology and Biological Sciences Research Council","NIGMS NIH HHS"],"pubmed_abstract":["STED microscopy is widely used to image subcellular structures with super-resolution. Here, we report that denoising STED images with deep learning can mitigate photobleaching and photodamage by reducing the pixel dwell time by one or two orders of magnitude. Our method allows for efficient and robust restoration of noisy 2D and 3D STED images with multiple targets and facilitates long-term imaging of mitochondrial dynamics."],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2023.01.26.525571"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9900922"],"repository":["biostudies-literature"],"pubmed_title":["Deep learning enables fast, gentle STED microscopy."],"pmcid":["PMC9900922"],"funding_grant_id":["R35 GM138039","MC_UU_00008/9","U01 DK127422","MC_UU_12010/9","BB/P026354/1","835102"],"pubmed_authors":["Eggeling C","Kim J","Jakobs S","Ebrahimi V","Carravilla P","Stephan T","Han KY"],"additional_accession":[]},"is_claimable":false,"name":"Deep learning enables fast, gentle STED microscopy.","description":"STED microscopy is widely used to image subcellular structures with super-resolution. Here, we report that denoising STED images with deep learning can mitigate photobleaching and photodamage by reducing the pixel dwell time by one or two orders of magnitude. Our method allows for efficient and robust restoration of noisy 2D and 3D STED images with multiple targets and facilitates long-term imaging of mitochondrial dynamics.","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jan","modification":"2025-04-04T13:08:13.8Z","creation":"2025-04-04T13:08:13.8Z"},"accession":"S-EPMC9900922","cross_references":{"pubmed":["36747618"],"doi":["10.1101/2023.01.26.525571"]}}