{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Lee JP"],"funding":["Ministry of Science and ICT, South Korea"],"pubmed_abstract":["Ginkgo biloba is utilized as food, medicine, wood, and street trees among other things. The objective of this study was to develop a loop-mediated isothermal amplification (LAMP) assay for gender distinction of G. biloba. Male-specific SCAR gene can be utilized to identify G. biloba gender using LAMP. The optimized LAMP conditions, temperature 60 °C, 2-mM MgSO<sub>4</sub>, and [F3/B3]:[FIP/BIP] primer ratio of 1:4 were selected as final conditions. The G. biloba SCAR LAMP displayed a sensitivity of 10 ng when amplified by concentration under the optimum conditions. Additionally, it demonstrated a particular response in male with SYBR Green I in LAMP analysis that can be a more powerful tool for field and scale-up applications. Our work represents a first attempt to identify G. biloba gende"],"journal":["Molecular biotechnology"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9901403"],"repository":["biostudies-literature"],"pubmed_title":["Distinction of Male and Female Trees of Ginkgo biloba Using LAMP."],"pmcid":["PMC9901403"],"funding_grant_id":["2020R1A2C1009463"],"pubmed_authors":["Park YS","Kim HK","Woo JA","Kim YH","Shin WR","Lee JP","Ahn JY"],"additional_accession":[]},"is_claimable":false,"name":"Distinction of Male and Female Trees of Ginkgo biloba Using LAMP.","description":"Ginkgo biloba is utilized as food, medicine, wood, and street trees among other things. The objective of this study was to develop a loop-mediated isothermal amplification (LAMP) assay for gender distinction of G. biloba. Male-specific SCAR gene can be utilized to identify G. biloba gender using LAMP. The optimized LAMP conditions, temperature 60 °C, 2-mM MgSO<sub>4</sub>, and [F3/B3]:[FIP/BIP] primer ratio of 1:4 were selected as final conditions. The G. biloba SCAR LAMP displayed a sensitivity of 10 ng when amplified by concentration under the optimum conditions. Additionally, it demonstrated a particular response in male with SYBR Green I in LAMP analysis that can be a more powerful tool for field and scale-up applications. Our work represents a first attempt to identify G. biloba gende","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Feb","modification":"2025-04-04T08:31:29.961Z","creation":"2025-04-04T08:31:29.961Z"},"accession":"S-EPMC9901403","cross_references":{"pubmed":["36745281"],"doi":["10.1007/s12033-023-00673-7"]}}