biostudies-literatureUnknownLee JPMinistry of Science and ICT, South KoreaGinkgo biloba is utilized as food, medicine, wood, and street trees among other things. The objective of this study was to develop a loop-mediated isothermal amplification (LAMP) assay for gender distinction of G. biloba. Male-specific SCAR gene can be utilized to identify G. biloba gender using LAMP. The optimized LAMP conditions, temperature 60 °C, 2-mM MgSO₄, and [F3/B3]:[FIP/BIP] primer ratio of 1:4 were selected as final conditions. The G. biloba SCAR LAMP displayed a sensitivity of 10 ng when amplified by concentration under the optimum conditions. Additionally, it demonstrated a particular response in male with SYBR Green I in LAMP analysis that can be a more powerful tool for field and scale-up applications. Our work represents a first attempt to identify G. biloba gendeMolecular biotechnologyhttps://www.ebi.ac.uk/biostudies/studies/S-EPMC9901403biostudies-literatureDistinction of Male and Female Trees of Ginkgo biloba Using LAMP.PMC99014032020R1A2C1009463Park YSKim HKWoo JAKim YHShin WRLee JPAhn JYfalseDistinction of Male and Female Trees of Ginkgo biloba Using LAMP.Ginkgo biloba is utilized as food, medicine, wood, and street trees among other things. The objective of this study was to develop a loop-mediated isothermal amplification (LAMP) assay for gender distinction of G. biloba. Male-specific SCAR gene can be utilized to identify G. biloba gender using LAMP. The optimized LAMP conditions, temperature 60 °C, 2-mM MgSO₄, and [F3/B3]:[FIP/BIP] primer ratio of 1:4 were selected as final conditions. The G. biloba SCAR LAMP displayed a sensitivity of 10 ng when amplified by concentration under the optimum conditions. Additionally, it demonstrated a particular response in male with SYBR Green I in LAMP analysis that can be a more powerful tool for field and scale-up applications. Our work represents a first attempt to identify G. biloba gende2023-01-01T00:00:00Z2023 Feb2025-04-04T08:31:29.961Z2025-04-04T08:31:29.961ZS-EPMC99014033674528110.1007/s12033-023-00673-7