{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Nguyen TTM"],"funding":["National Research Foundation (KR)","National Research Foundation"],"pagination":["42"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9903620"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["42(1)"],"pubmed_abstract":["<h4>Background</h4>Clear cell renal cell carcinoma (ccRCC), with its hallmark phenotype of high cytosolic lipid content, is considered a metabolic cancer. Despite the implication of this lipid-rich phenotype in ccRCC tumorigenesis, the roles and regulators of de novo lipid synthesis (DNL) in ccRCC remain largely unexplained.<h4>Methods</h4>Our bioinformatic screening focused on ccRCC-lipid phenotypes identified glutathione peroxidase 8 (GPX8), as a clinically relevant upstream regulator of DNL. GPX8 genetic silencing was performed with CRISPR-Cas9 or shRNA in ccRCC cell lines to dissect its roles. Untargeted metabolomics, RNA-seq analyses, and other biochemical assays (e.g., lipid droplets staining, fatty acid uptake, cell proliferation, xenograft, etc.) were carried out to investigate the"],"journal":["Journal of experimental & clinical cancer research : CR"],"pubmed_title":["GPX8 regulates clear cell renal cell carcinoma tumorigenesis through promoting lipogenesis by NNMT."],"pmcid":["PMC9903620"],"funding_grant_id":["NRF-2020R1I1A1A01073124","NRF-2018R1A3B1052328"],"pubmed_authors":["Mai VH","Kim JM","An YJ","Nguyen TH","Kang S","Kim HS","Jung CR","Dao TTP","Seo M","Park S","Nguyen TTM","Moon Y"],"additional_accession":[]},"is_claimable":false,"name":"GPX8 regulates clear cell renal cell carcinoma tumorigenesis through promoting lipogenesis by NNMT.","description":"<h4>Background</h4>Clear cell renal cell carcinoma (ccRCC), with its hallmark phenotype of high cytosolic lipid content, is considered a metabolic cancer. Despite the implication of this lipid-rich phenotype in ccRCC tumorigenesis, the roles and regulators of de novo lipid synthesis (DNL) in ccRCC remain largely unexplained.<h4>Methods</h4>Our bioinformatic screening focused on ccRCC-lipid phenotypes identified glutathione peroxidase 8 (GPX8), as a clinically relevant upstream regulator of DNL. GPX8 genetic silencing was performed with CRISPR-Cas9 or shRNA in ccRCC cell lines to dissect its roles. Untargeted metabolomics, RNA-seq analyses, and other biochemical assays (e.g., lipid droplets staining, fatty acid uptake, cell proliferation, xenograft, etc.) were carried out to investigate the","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Feb","modification":"2025-05-29T19:43:41.846Z","creation":"2025-04-04T12:55:55.586Z"},"accession":"S-EPMC9903620","cross_references":{"pubmed":["36750850"],"doi":["10.1186/s13046-023-02607-2"]}}