{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["14"],"submitter":["Ye J"],"pubmed_abstract":["<h4>Objectives</h4>C1s activation is associated with the pathogenesis of various diseases, indicating the potential value of C1s activation detection in clinic. Here we aimed to establish fluorescence resonance energy transfer (FRET)-based immunoassay for the quantitative detection of activated C1s in serum.<h4>Methods</h4>FRET-based fluorogenic peptides, sensitive to the enzymatic activity of activated C1s, were prepared and labeled with the fluorophore ortho-aminobenzoic acid (Abz) and quencher 2,4-dinitrophenyl (Dnp), and then were further selected depending on its Kcat/Km value. C1s in the samples was captured and separated using anti-C1s-conjugated magnetic microbeads. Next, enzymatic activity of activated C1s in samples and standards was examined using fluorescent quenched substrate "],"journal":["Frontiers in immunology"],"pagination":["1081793"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9904206"],"repository":["biostudies-literature"],"pubmed_title":["Quantitative fluorescence resonance energy transfer-based immunoassay for activated complement C1s."],"pmcid":["PMC9904206"],"pubmed_authors":["Zhu L","Xia S","Zhang C","Xu J","Ye J"],"additional_accession":[]},"is_claimable":false,"name":"Quantitative fluorescence resonance energy transfer-based immunoassay for activated complement C1s.","description":"<h4>Objectives</h4>C1s activation is associated with the pathogenesis of various diseases, indicating the potential value of C1s activation detection in clinic. Here we aimed to establish fluorescence resonance energy transfer (FRET)-based immunoassay for the quantitative detection of activated C1s in serum.<h4>Methods</h4>FRET-based fluorogenic peptides, sensitive to the enzymatic activity of activated C1s, were prepared and labeled with the fluorophore ortho-aminobenzoic acid (Abz) and quencher 2,4-dinitrophenyl (Dnp), and then were further selected depending on its Kcat/Km value. C1s in the samples was captured and separated using anti-C1s-conjugated magnetic microbeads. Next, enzymatic activity of activated C1s in samples and standards was examined using fluorescent quenched substrate ","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023","modification":"2025-04-04T18:43:46.888Z","creation":"2025-04-04T18:43:46.888Z"},"accession":"S-EPMC9904206","cross_references":{"pubmed":["36761732"],"doi":["10.3389/fimmu.2023.1081793"]}}