biostudies-literatureUnknown10Yao S<h4>Background</h4>Efficiently delivering nucleic acid into mammalian cells is essential to overexpress genes for assessing gene functions. Human bone marrow stem cells (hBMSCs) are the most studied tissue-derived stem cells. Adeno-associated viruses (AAVs) have been used to deliver DNA into hBMSCs for various purposes. Current literature reported that transduction efficiencies of up to 65% could be achieved by AAV gene delivery into hBMSCs. Further improvement of efficiency is needed and possible. This study tested a selection of AAV serotypes for high-efficient DNA delivery into hBMSCs.<h4>Methods</h4>hBMSCs from different donors were infected with different serotypes of AAVs containing the enhanced green fluorescence protein (<i>eGFP</i>) reporter gene driven by the CMV promoter. Green Stem cell investigation3https://www.ebi.ac.uk/biostudies/studies/S-EPMC9905037biostudies-literatureOptimization of adeno-associated virus (AAV) gene delivery into human bone marrow stem cells (hBMSCs).PMC9905037Rong WYao SYuan YfalseOptimization of adeno-associated virus (AAV) gene delivery into human bone marrow stem cells (hBMSCs).<h4>Background</h4>Efficiently delivering nucleic acid into mammalian cells is essential to overexpress genes for assessing gene functions. Human bone marrow stem cells (hBMSCs) are the most studied tissue-derived stem cells. Adeno-associated viruses (AAVs) have been used to deliver DNA into hBMSCs for various purposes. Current literature reported that transduction efficiencies of up to 65% could be achieved by AAV gene delivery into hBMSCs. Further improvement of efficiency is needed and possible. This study tested a selection of AAV serotypes for high-efficient DNA delivery into hBMSCs.<h4>Methods</h4>hBMSCs from different donors were infected with different serotypes of AAVs containing the enhanced green fluorescence protein (<i>eGFP</i>) reporter gene driven by the CMV promoter. Green 2023-01-01T00:00:00Z20232025-04-04T18:43:59.637Z2025-04-04T18:43:59.637ZS-EPMC99050373676125310.21037/sci-2022-042