{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Rueter J"],"funding":["Deutsche Forschungsgemeinschaft","Christian-Albrechts-Universität zu Kiel"],"pagination":["59"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9905200"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["80(3)"],"pubmed_abstract":["<h4>Background and aims</h4>Apolipoprotein E (APOE) is known for its role in lipid metabolism and its association with age-related disease pathology. The aim of the present work was to identify previously unknown functions of APOE based on the detection of novel APOE protein-protein interaction candidates.<h4>Approach and results</h4>APOE targeted replacement mice and transfected cultured hepatocytes expressing the human isoforms APOE3 and APOE4 were used. For 7 months, APOE3 and APOE4 mice were fed a high-fat and high-sugar diet to induce obesity, while a subgroup was subjected to 30% dietary restriction. Proteomic analysis of coimmunoprecipitation products from APOE mouse liver extracts revealed 28 APOE-interacting candidate proteins, including branched-chain alpha-keto acid dehydrogenas"],"journal":["Cellular and molecular life sciences : CMLS"],"pubmed_title":["The mitochondrial BCKD complex interacts with hepatic apolipoprotein E in cultured cells in vitro and mouse livers in vivo."],"pmcid":["PMC9905200"],"funding_grant_id":["HU 1702/6-1","TH 872/13-1"],"pubmed_authors":["Tholey A","Huebbe P","Treitz C","Rueter J","Luersen K","Rimbach G","Schloesser A"],"additional_accession":[]},"is_claimable":false,"name":"The mitochondrial BCKD complex interacts with hepatic apolipoprotein E in cultured cells in vitro and mouse livers in vivo.","description":"<h4>Background and aims</h4>Apolipoprotein E (APOE) is known for its role in lipid metabolism and its association with age-related disease pathology. The aim of the present work was to identify previously unknown functions of APOE based on the detection of novel APOE protein-protein interaction candidates.<h4>Approach and results</h4>APOE targeted replacement mice and transfected cultured hepatocytes expressing the human isoforms APOE3 and APOE4 were used. For 7 months, APOE3 and APOE4 mice were fed a high-fat and high-sugar diet to induce obesity, while a subgroup was subjected to 30% dietary restriction. Proteomic analysis of coimmunoprecipitation products from APOE mouse liver extracts revealed 28 APOE-interacting candidate proteins, including branched-chain alpha-keto acid dehydrogenas","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Feb","modification":"2026-07-14T19:06:05.688Z","creation":"2024-11-20T01:39:21.053Z"},"accession":"S-EPMC9905200","cross_references":{"pubmed":["36749362"],"doi":["10.1007/s00018-023-04706-x"]}}