biostudies-literatureRueter JDeutsche ForschungsgemeinschaftChristian-Albrechts-Universität zu Kiel59https://www.ebi.ac.uk/biostudies/studies/S-EPMC9905200biostudies-literatureUnknown80(3)<h4>Background and aims</h4>Apolipoprotein E (APOE) is known for its role in lipid metabolism and its association with age-related disease pathology. The aim of the present work was to identify previously unknown functions of APOE based on the detection of novel APOE protein-protein interaction candidates.<h4>Approach and results</h4>APOE targeted replacement mice and transfected cultured hepatocytes expressing the human isoforms APOE3 and APOE4 were used. For 7 months, APOE3 and APOE4 mice were fed a high-fat and high-sugar diet to induce obesity, while a subgroup was subjected to 30% dietary restriction. Proteomic analysis of coimmunoprecipitation products from APOE mouse liver extracts revealed 28 APOE-interacting candidate proteins, including branched-chain alpha-keto acid dehydrogenasCellular and molecular life sciences : CMLSThe mitochondrial BCKD complex interacts with hepatic apolipoprotein E in cultured cells in vitro and mouse livers in vivo.PMC9905200HU 1702/6-1TH 872/13-1Tholey AHuebbe PTreitz CRueter JLuersen KRimbach GSchloesser AfalseThe mitochondrial BCKD complex interacts with hepatic apolipoprotein E in cultured cells in vitro and mouse livers in vivo.<h4>Background and aims</h4>Apolipoprotein E (APOE) is known for its role in lipid metabolism and its association with age-related disease pathology. The aim of the present work was to identify previously unknown functions of APOE based on the detection of novel APOE protein-protein interaction candidates.<h4>Approach and results</h4>APOE targeted replacement mice and transfected cultured hepatocytes expressing the human isoforms APOE3 and APOE4 were used. For 7 months, APOE3 and APOE4 mice were fed a high-fat and high-sugar diet to induce obesity, while a subgroup was subjected to 30% dietary restriction. Proteomic analysis of coimmunoprecipitation products from APOE mouse liver extracts revealed 28 APOE-interacting candidate proteins, including branched-chain alpha-keto acid dehydrogenas2023-01-01T00:00:00Z2023 Feb2026-07-14T19:06:05.688Z2024-11-20T01:39:21.053ZS-EPMC99052003674936210.1007/s00018-023-04706-x