{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13"],"submitter":["Peiffer L"],"pubmed_abstract":["<h4>Background</h4>Sézary Syndrome (SS) is an aggressive leukemic variant of cutaneous T-cell lymphomas (CTCL). In SS patients, malignant T cells are circulating through the blood and cause erythroderma.<h4>Objective</h4>To compare the transcriptome of single cells in blood and skin samples from a patient with advanced SS.<h4>Methods</h4>We utilized combined single cell RNA and T-cell receptor (TCR) sequencing (scRNA-seq).<h4>Results</h4>We scrutinized the malignant T cells in blood and skin in an unbiased manner without pre-sorting of cells. We observed different phenotypes of the same monoclonal malignant T-cell population, confirmed by TCR sequencing and inferred copy number variation analysis. Malignant T cells present in the circulating blood expressed genes resembling central memory "],"journal":["Frontiers in oncology"],"pagination":["1090592"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9905421"],"repository":["biostudies-literature"],"pubmed_title":["Phenotypic plasticity of malignant T cells in blood and skin of a Sezary syndrome patient revealed by single cell transcriptomics."],"pmcid":["PMC9905421"],"pubmed_authors":["Stranzenbach R","Horny K","Gambichler T","Susok L","Peiffer L","Spassova I","Furtmann F","Gravemeyer J","Buus TB","Kubat L","Srinivas N","Odum N","Becker JC"],"additional_accession":[]},"is_claimable":false,"name":"Phenotypic plasticity of malignant T cells in blood and skin of a Sezary syndrome patient revealed by single cell transcriptomics.","description":"<h4>Background</h4>Sézary Syndrome (SS) is an aggressive leukemic variant of cutaneous T-cell lymphomas (CTCL). In SS patients, malignant T cells are circulating through the blood and cause erythroderma.<h4>Objective</h4>To compare the transcriptome of single cells in blood and skin samples from a patient with advanced SS.<h4>Methods</h4>We utilized combined single cell RNA and T-cell receptor (TCR) sequencing (scRNA-seq).<h4>Results</h4>We scrutinized the malignant T cells in blood and skin in an unbiased manner without pre-sorting of cells. We observed different phenotypes of the same monoclonal malignant T-cell population, confirmed by TCR sequencing and inferred copy number variation analysis. Malignant T cells present in the circulating blood expressed genes resembling central memory ","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023","modification":"2025-04-18T21:17:00.246Z","creation":"2025-04-07T09:16:31.596Z"},"accession":"S-EPMC9905421","cross_references":{"pubmed":["36761972"],"doi":["10.3389/fonc.2023.1090592"]}}