biostudies-literatureUnknown13Peiffer L<h4>Background</h4>Sézary Syndrome (SS) is an aggressive leukemic variant of cutaneous T-cell lymphomas (CTCL). In SS patients, malignant T cells are circulating through the blood and cause erythroderma.<h4>Objective</h4>To compare the transcriptome of single cells in blood and skin samples from a patient with advanced SS.<h4>Methods</h4>We utilized combined single cell RNA and T-cell receptor (TCR) sequencing (scRNA-seq).<h4>Results</h4>We scrutinized the malignant T cells in blood and skin in an unbiased manner without pre-sorting of cells. We observed different phenotypes of the same monoclonal malignant T-cell population, confirmed by TCR sequencing and inferred copy number variation analysis. Malignant T cells present in the circulating blood expressed genes resembling central memory Frontiers in oncology1090592https://www.ebi.ac.uk/biostudies/studies/S-EPMC9905421biostudies-literaturePhenotypic plasticity of malignant T cells in blood and skin of a Sezary syndrome patient revealed by single cell transcriptomics.PMC9905421Stranzenbach RHorny KGambichler TSusok LPeiffer LSpassova IFurtmann FGravemeyer JBuus TBKubat LSrinivas NOdum NBecker JCfalsePhenotypic plasticity of malignant T cells in blood and skin of a Sezary syndrome patient revealed by single cell transcriptomics.<h4>Background</h4>Sézary Syndrome (SS) is an aggressive leukemic variant of cutaneous T-cell lymphomas (CTCL). In SS patients, malignant T cells are circulating through the blood and cause erythroderma.<h4>Objective</h4>To compare the transcriptome of single cells in blood and skin samples from a patient with advanced SS.<h4>Methods</h4>We utilized combined single cell RNA and T-cell receptor (TCR) sequencing (scRNA-seq).<h4>Results</h4>We scrutinized the malignant T cells in blood and skin in an unbiased manner without pre-sorting of cells. We observed different phenotypes of the same monoclonal malignant T-cell population, confirmed by TCR sequencing and inferred copy number variation analysis. Malignant T cells present in the circulating blood expressed genes resembling central memory 2023-01-01T00:00:00Z20232025-04-18T21:17:00.246Z2025-04-07T09:16:31.596ZS-EPMC99054213676197210.3389/fonc.2023.1090592