biostudies-literatureUnknown9(2)Kawarasaki TThe ubiquitin-specific chaperone AAA-ATPase Cdc48 and its orthologs p97/valosin-containing protein (VCP) in mammals play crucial roles in regulating numerous intracellular pathways via segregase activity, which separates polyubiquitinated targets from membranes or binding partners. Interestingly, high-throughput experiments show that a vast number of metabolic enzymes are modified with ubiquitin. Therefore, Cdc48 may regulate metabolic pathways, for example by acting on the polyubiquitin chains of metabolic enzymes; however, the role of Cdc48 in metabolic regulation remains largely unknown. To begin to analyze the role of Cdc48 in metabolic regulation in yeast, we performed a metabolomics analysis of temperature-sensitive <i>cdc48-3</i> mutant cells. We found that the amount of metabolitesHeliyone13219https://www.ebi.ac.uk/biostudies/studies/S-EPMC9905943biostudies-literatureMetabolomics analysis of an AAA-ATPase Cdc48-deficient yeast strain.PMC9905943Kawarasaki TNakatsukasa KfalseMetabolomics analysis of an AAA-ATPase Cdc48-deficient yeast strain.The ubiquitin-specific chaperone AAA-ATPase Cdc48 and its orthologs p97/valosin-containing protein (VCP) in mammals play crucial roles in regulating numerous intracellular pathways via segregase activity, which separates polyubiquitinated targets from membranes or binding partners. Interestingly, high-throughput experiments show that a vast number of metabolic enzymes are modified with ubiquitin. Therefore, Cdc48 may regulate metabolic pathways, for example by acting on the polyubiquitin chains of metabolic enzymes; however, the role of Cdc48 in metabolic regulation remains largely unknown. To begin to analyze the role of Cdc48 in metabolic regulation in yeast, we performed a metabolomics analysis of temperature-sensitive <i>cdc48-3</i> mutant cells. We found that the amount of metabolites2023-01-01T00:00:00Z2023 Feb2025-04-18T21:16:42.007Z2025-04-07T09:14:29.32ZS-EPMC99059433676182610.1016/j.heliyon.2023.e13219