biostudies-literatureUnknown11(1)Ren S<h4>Background</h4>Keloid is a dermal fibrotic disease characterized by excessive proliferation of dermal fibroblasts and deposition of excessive collagen. N6-methyladenosine (m6A) plays a significant role in numerous physiological and pathological regulatory processes in the human body. Fat mass and obesity-associated protein (FTO) is one of the most essential m6A demethylases. However, whether FTO has a regulatory role in keloid development remains to be determined.<h4>Methods</h4>In this study, we investigated the effects of the m6A demethylase FTO on keloid formation by performing hematoxylin and eosin (H&E) staining, m6A dot blotting, transwell migration experiment, and methylated RNA immunoprecipitation quantitative polymerase chain reaction (MeRIP-qPCR) tests, as well as real-time PAnnals of translational medicine15https://www.ebi.ac.uk/biostudies/studies/S-EPMC9906204biostudies-literatureThe m6A demethylase FTO promotes keloid formation by up-regulating COL1A1.PMC9906204Wang MYe MJi YHuang LRen SCai XfalseThe m6A demethylase FTO promotes keloid formation by up-regulating COL1A1.<h4>Background</h4>Keloid is a dermal fibrotic disease characterized by excessive proliferation of dermal fibroblasts and deposition of excessive collagen. N6-methyladenosine (m6A) plays a significant role in numerous physiological and pathological regulatory processes in the human body. Fat mass and obesity-associated protein (FTO) is one of the most essential m6A demethylases. However, whether FTO has a regulatory role in keloid development remains to be determined.<h4>Methods</h4>In this study, we investigated the effects of the m6A demethylase FTO on keloid formation by performing hematoxylin and eosin (H&E) staining, m6A dot blotting, transwell migration experiment, and methylated RNA immunoprecipitation quantitative polymerase chain reaction (MeRIP-qPCR) tests, as well as real-time P2023-01-01T00:00:00Z2023 Jan2025-04-18T21:16:29.375Z2025-04-07T09:16:18.651ZS-EPMC99062043676023810.21037/atm-22-6021