{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Lup SD"],"funding":["Ministerio de Ciencia e Innovación","Generalitat Valenciana"],"pagination":["1042913"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9909543"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14"],"pubmed_abstract":["Mapping-by-sequencing combines Next Generation Sequencing (NGS) with classical genetic mapping by linkage analysis to establish gene-to-phenotype relationships. Although numerous tools have been developed to analyze NGS datasets, only a few are available for mapping-by-sequencing. One such tool is Easymap, a versatile, easy-to-use package that performs automated mapping of point mutations and large DNA insertions. Here, we describe Easymap v.2, which also maps small insertion/deletions (InDels), and includes workflows to perform QTL-seq and variant density mapping analyses. Each mapping workflow can accommodate different experimental designs, including outcrossing and backcrossing, F<sub>2</sub>, M<sub>2</sub>, and M<sub>3</sub> mapping populations, chemically induced mutation and natural "],"journal":["Frontiers in plant science"],"pubmed_title":["Versatile mapping-by-sequencing with Easymap v.2."],"pmcid":["PMC9909543"],"funding_grant_id":["PROMETEO/2019/117, ACIF/2018/005","PGC2018-093445-B-I00, PID2021-127725NB-I00"],"pubmed_authors":["Micol JL","Navarro-Quiles C","Lup SD"],"additional_accession":[]},"is_claimable":false,"name":"Versatile mapping-by-sequencing with Easymap v.2.","description":"Mapping-by-sequencing combines Next Generation Sequencing (NGS) with classical genetic mapping by linkage analysis to establish gene-to-phenotype relationships. Although numerous tools have been developed to analyze NGS datasets, only a few are available for mapping-by-sequencing. One such tool is Easymap, a versatile, easy-to-use package that performs automated mapping of point mutations and large DNA insertions. Here, we describe Easymap v.2, which also maps small insertion/deletions (InDels), and includes workflows to perform QTL-seq and variant density mapping analyses. Each mapping workflow can accommodate different experimental designs, including outcrossing and backcrossing, F<sub>2</sub>, M<sub>2</sub>, and M<sub>3</sub> mapping populations, chemically induced mutation and natural ","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023","modification":"2026-07-14T18:40:42.766Z","creation":"2024-11-09T01:30:26.794Z"},"accession":"S-EPMC9909543","cross_references":{"pubmed":["36778692"],"doi":["10.3389/fpls.2023.1042913"]}}