biostudies-literatureUnknown19(3)Chen CCardiac fibrosis after myocardial ischemic (MI) injury is a key factor in heart function deterioration. We recently showed that ubiquitin-like protein human HLA-F adjacent transcript (FAT10) plays a novel role in ischemic cardiovascular diseases, but its function in cardiac fibrosis remains unknown. The present study aims to detail the pathophysiological function of FAT10 in MI injury-induced cardiac fibrosis and its underlying mechanism. <i>In vivo</i>, a systemic FAT10 deficiency mouse (<i>Fat10</i> ^-/-) model was established which exhibited excessive cardiac fibrosis and deleterious cardiac function after MI when compared to wild-type mice. Cardiac fibrotic-related proteins (α-SMA, collagen I and collagen III) content were increased in MI-<i>Fat10</i> ^-/- mice. SimInternational journal of biological sciences881-896https://www.ebi.ac.uk/biostudies/studies/S-EPMC9910007biostudies-literatureUbiquitin like protein FAT10 repressed cardiac fibrosis after myocardial ischemic via mediating degradation of Smad3 dependent on FAT10-proteasome system.PMC9910007Li XWan RYu BXie JHong KZhou TChen CSu YShen YJin JfalseUbiquitin like protein FAT10 repressed cardiac fibrosis after myocardial ischemic via mediating degradation of Smad3 dependent on FAT10-proteasome system.Cardiac fibrosis after myocardial ischemic (MI) injury is a key factor in heart function deterioration. We recently showed that ubiquitin-like protein human HLA-F adjacent transcript (FAT10) plays a novel role in ischemic cardiovascular diseases, but its function in cardiac fibrosis remains unknown. The present study aims to detail the pathophysiological function of FAT10 in MI injury-induced cardiac fibrosis and its underlying mechanism. <i>In vivo</i>, a systemic FAT10 deficiency mouse (<i>Fat10</i> ^-/-) model was established which exhibited excessive cardiac fibrosis and deleterious cardiac function after MI when compared to wild-type mice. Cardiac fibrotic-related proteins (α-SMA, collagen I and collagen III) content were increased in MI-<i>Fat10</i> ^-/- mice. Sim2023-01-01T00:00:00Z20232025-05-29T16:30:18.856Z2025-04-04T23:57:09.059ZS-EPMC99100073677811410.7150/ijbs.77677