biostudies-literatureMokashi SSNIGMS NIH HHSjkac307https://www.ebi.ac.uk/biostudies/studies/S-EPMC9911060biostudies-literatureUnknown13(2)Insect odorant-binding proteins (OBPs) are members of a rapidly evolving multigene family traditionally thought to facilitate chemosensation. However, studies on Drosophila have shown that members of this family have evolved functions beyond chemosensation, as evident from their expression in reproductive tissues and the brain. Previous studies implicated diverse functions of Obp56h, a member of the largest gene cluster of the D. melanogaster Obp repertoire. Here, we examined the effect of CRISPR/Cas9-mediated deletion of Obp56h on 2 fitness phenotypes, on resistance to starvation stress and heat stress, and on locomotion and sleep phenotypes. Obp56h-/- mutants show a strong sexually dimorphic effect on starvation stress survival, with females being more resistant to starvation stress thanG3 (Bethesda, Md.)Pleiotropic fitness effects of a Drosophila odorant-binding protein.PMC9911060P20 GM139769R01 GM059469R01 GM128974Mokashi SSJohnstun JAMackay TFCShankar VAnholt RRHfalsePleiotropic fitness effects of a Drosophila odorant-binding protein.Insect odorant-binding proteins (OBPs) are members of a rapidly evolving multigene family traditionally thought to facilitate chemosensation. However, studies on Drosophila have shown that members of this family have evolved functions beyond chemosensation, as evident from their expression in reproductive tissues and the brain. Previous studies implicated diverse functions of Obp56h, a member of the largest gene cluster of the D. melanogaster Obp repertoire. Here, we examined the effect of CRISPR/Cas9-mediated deletion of Obp56h on 2 fitness phenotypes, on resistance to starvation stress and heat stress, and on locomotion and sleep phenotypes. Obp56h-/- mutants show a strong sexually dimorphic effect on starvation stress survival, with females being more resistant to starvation stress than2023-01-01T00:00:00Z2023 Feb2025-04-04T18:42:22.329Z2025-04-04T18:42:22.329ZS-EPMC99110603645409810.1093/g3journal/jkac307