biostudies-literatureUnknown14Wu RNational Natural Science Foundation of ChinaHexokinase1 (HXK1) is a bifunctional enzyme that plays indispensable roles in plant growth, nitrogen utilization, and stress resistance. However, information on the HXK family members of strawberries and their functions in glucose sensing and metabolic regulation is scarce. In the present study, four HXKs were firstly identified in the genome of <i>Fragaria vesca</i> and <i>F. pentaphylla</i>. The conserved domains of the HXK1s were confirmed, and a site-directed mutation (S177A) was introduced into the FpHXK1. <i>FpHXK1</i>, which shares the highest identity with the <i>AtHXK1</i> was able to restore the glucose sensitivity and developmental defects of the Arabidopsis <i>gin2-1</i> mutant, but not its kinase-activity-impaired mutant (<i>FpHXK1^S177A</i> ). The transcription of <Frontiers in plant science1069830https://www.ebi.ac.uk/biostudies/studies/S-EPMC9911861biostudies-literatureHexokinase1: A glucose sensor involved in drought stress response and sugar metabolism depending on its kinase activity in strawberry.PMC9911861Chen QHe JLin XLin YLi MTang HZhang YHe WPang LWu RLuo YWang XWang YMin AfalseHexokinase1: A glucose sensor involved in drought stress response and sugar metabolism depending on its kinase activity in strawberry.Hexokinase1 (HXK1) is a bifunctional enzyme that plays indispensable roles in plant growth, nitrogen utilization, and stress resistance. However, information on the HXK family members of strawberries and their functions in glucose sensing and metabolic regulation is scarce. In the present study, four HXKs were firstly identified in the genome of <i>Fragaria vesca</i> and <i>F. pentaphylla</i>. The conserved domains of the HXK1s were confirmed, and a site-directed mutation (S177A) was introduced into the FpHXK1. <i>FpHXK1</i>, which shares the highest identity with the <i>AtHXK1</i> was able to restore the glucose sensitivity and developmental defects of the Arabidopsis <i>gin2-1</i> mutant, but not its kinase-activity-impaired mutant (<i>FpHXK1^S177A</i> ). The transcription of <2023-01-01T00:00:00Z20232026-05-29T02:22:34.484Z2025-02-19T02:23:53.012ZS-EPMC99118613677869110.3389/fpls.2023.1069830