biostudies-literatureUnknown14Tojo HMinistry of Education, Culture, Sports, Science and TechnologyJapan Society for the Promotion of ScienceThe regulation of intracellular pyrophosphate (PPi) level is crucial for proper morphogenesis across all taxonomic kingdoms. PPi is released as a byproduct from ~200 metabolic reactions, then hydrolyzed by either membrane-bound (H⁺-PPase) or soluble pyrophosphatases (PPases). In Arabidopsis, the loss of the vacuolar H⁺-PPase/FUGU5, a key enzyme in PPi homeostasis, results in delayed growth and a number of developmental defects, pointing to the importance of PPi homeostasis in plant morphogenesis. The Arabidopsis genome encodes several PPases in addition to FUGU5, such as PPsPase1/PECP2, VHP2;1 and VHP2;2, although their significance regarding PPi homeostasis remains elusive. Here, to assess their contribution, phenotypic analyses of cotyledon aspect ratio, palisade tiFrontiers in plant science1031426https://www.ebi.ac.uk/biostudies/studies/S-EPMC9911876biostudies-literatureRoles of type II H⁺-PPases and PPsPase1/PECP2 in early developmental stages and PPi homeostasis of <i>Arabidopsis thaliana</i>.PMC9911876Hirai MYGunji SDavid PFerjani ATabeta HJavot HTojo HfalseRoles of type II H⁺-PPases and PPsPase1/PECP2 in early developmental stages and PPi homeostasis of <i>Arabidopsis thaliana</i>.The regulation of intracellular pyrophosphate (PPi) level is crucial for proper morphogenesis across all taxonomic kingdoms. PPi is released as a byproduct from ~200 metabolic reactions, then hydrolyzed by either membrane-bound (H⁺-PPase) or soluble pyrophosphatases (PPases). In Arabidopsis, the loss of the vacuolar H⁺-PPase/FUGU5, a key enzyme in PPi homeostasis, results in delayed growth and a number of developmental defects, pointing to the importance of PPi homeostasis in plant morphogenesis. The Arabidopsis genome encodes several PPases in addition to FUGU5, such as PPsPase1/PECP2, VHP2;1 and VHP2;2, although their significance regarding PPi homeostasis remains elusive. Here, to assess their contribution, phenotypic analyses of cotyledon aspect ratio, palisade ti2023-01-01T00:00:00Z20232026-04-08T14:16:13.743Z2025-02-19T02:23:59.282ZS-EPMC99118763677868810.3389/fpls.2023.1031426