biostudies-literatureHauschulz MDeutsche Forschungsgemeinschaft683https://www.ebi.ac.uk/biostudies/studies/S-EPMC9913221biostudies-literatureUnknown15(3)In pancreatic cancer treatment, tumor stage-dependent chemotherapies are used to prolong overall survival. By measuring DNA promoter hypermethylation in the plasma of patients with stage IV pancreatic cancer, it was recently shown that promoter DNA methylation of the tumor suppressor gene <i>SFRP1</i> has a high value for predicting failure of drug treatment with gemcitabine. In this study, we therefore aimed to identify as precisely as possible the region in the <i>SFRP1</i> promoter that is frequently hypermethylated in pancreatic cancer tissue. First, we used the TCGA data set to define CpG-rich regions flanking the <i>SFRP1</i> transcription start site that were significantly more methylated in pancreatic cancer compared to normal pancreatic acinar tissue. A core CpG island was identifCancersIdentification and Validation of Potentially Clinically Relevant CpG Regions within the Class 2 Tumor Suppressor Gene <i>SFRP1</i> in Pancreatic Cancer.PMC9913221331065168Bednarsch JSteib FKosinski JKnuchel-Clarke RDahl EHeij LRHauschulz MVillwock SfalseIdentification and Validation of Potentially Clinically Relevant CpG Regions within the Class 2 Tumor Suppressor Gene <i>SFRP1</i> in Pancreatic Cancer.In pancreatic cancer treatment, tumor stage-dependent chemotherapies are used to prolong overall survival. By measuring DNA promoter hypermethylation in the plasma of patients with stage IV pancreatic cancer, it was recently shown that promoter DNA methylation of the tumor suppressor gene <i>SFRP1</i> has a high value for predicting failure of drug treatment with gemcitabine. In this study, we therefore aimed to identify as precisely as possible the region in the <i>SFRP1</i> promoter that is frequently hypermethylated in pancreatic cancer tissue. First, we used the TCGA data set to define CpG-rich regions flanking the <i>SFRP1</i> transcription start site that were significantly more methylated in pancreatic cancer compared to normal pancreatic acinar tissue. A core CpG island was identif2023-01-01T00:00:00Z2023 Jan2026-06-20T03:18:03.602Z2025-04-06T07:56:38.807ZS-EPMC99132213676563910.3390/cancers15030683