biostudies-literatureUnknown15(3)Tedja R<h4>Purpose</h4>Cancer progression, invasiveness, and metastatic potential have been associated with the activation of the cellular development program known as epithelial-to-mesenchymal transition (EMT). This process is known to yield not only mesenchymal cells, but instead an array of cells with different degrees of epithelial and mesenchymal phenotypes with high plasticity, usually referred to as E/M hybrid cells. The characteristics of E/M hybrid cells, their importance in tumor progression, and the key regulators in the tumor microenvironment that support this phenotype are still poorly understood.<h4>Methods</h4>In this study, we established an in vitro model of EMT and characterized the different stages of differentiation, allowing us to identify the main genomic signature associateCancers684https://www.ebi.ac.uk/biostudies/studies/S-EPMC9913490biostudies-literatureGeneration of Stable Epithelial-Mesenchymal Hybrid Cancer Cells with Tumorigenic Potential.PMC9913490Bawa TChehade HAdzibolosu NPitruzzello MMor GGogoi RTedja RAlvero ABFox ACardenas CfalseGeneration of Stable Epithelial-Mesenchymal Hybrid Cancer Cells with Tumorigenic Potential.<h4>Purpose</h4>Cancer progression, invasiveness, and metastatic potential have been associated with the activation of the cellular development program known as epithelial-to-mesenchymal transition (EMT). This process is known to yield not only mesenchymal cells, but instead an array of cells with different degrees of epithelial and mesenchymal phenotypes with high plasticity, usually referred to as E/M hybrid cells. The characteristics of E/M hybrid cells, their importance in tumor progression, and the key regulators in the tumor microenvironment that support this phenotype are still poorly understood.<h4>Methods</h4>In this study, we established an in vitro model of EMT and characterized the different stages of differentiation, allowing us to identify the main genomic signature associate2023-01-01T00:00:00Z2023 Jan2025-04-25T19:24:17.93Z2025-04-06T07:57:38.671ZS-EPMC99134903676564110.3390/cancers15030684