{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Varga-Zsiros V"],"funding":["Cancer Foundation Finland sr"],"pagination":["428"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9913738"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(3)"],"pubmed_abstract":["Lipid metabolic disturbances are associated with several diseases, such as type 2 diabetes or malignancy. In the last two decades, high-performance mass spectrometry-based lipidomics has emerged as a valuable tool in various fields of biology. However, the evaluation of macroscopic tissue homogenates leaves often undiscovered the differences arising from micron-scale heterogeneity. Therefore, in this work, we developed a novel laser microdissection-coupled shotgun lipidomic platform, which combines quantitative and broad-range lipidome analysis with reasonable spatial resolution. The multistep approach involves the preparation of successive cryosections from tissue samples, cross-referencing of native and stained images, laser microdissection of regions of interest, in situ lipid extractio"],"journal":["Cells"],"pubmed_title":["Development of a Laser Microdissection-Coupled Quantitative Shotgun Lipidomic Method to Uncover Spatial Heterogeneity."],"pmcid":["PMC9913738"],"funding_grant_id":["200082"],"pubmed_authors":["Marton A","Vizler C","Torok Z","Kota Z","Peter M","Varga-Zsiros V","Tiszlavicz L","Vigh L","Horvath P","Balogh G","Migh E"],"additional_accession":[]},"is_claimable":false,"name":"Development of a Laser Microdissection-Coupled Quantitative Shotgun Lipidomic Method to Uncover Spatial Heterogeneity.","description":"Lipid metabolic disturbances are associated with several diseases, such as type 2 diabetes or malignancy. In the last two decades, high-performance mass spectrometry-based lipidomics has emerged as a valuable tool in various fields of biology. However, the evaluation of macroscopic tissue homogenates leaves often undiscovered the differences arising from micron-scale heterogeneity. Therefore, in this work, we developed a novel laser microdissection-coupled shotgun lipidomic platform, which combines quantitative and broad-range lipidome analysis with reasonable spatial resolution. The multistep approach involves the preparation of successive cryosections from tissue samples, cross-referencing of native and stained images, laser microdissection of regions of interest, in situ lipid extractio","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jan","modification":"2025-04-22T14:46:31.856Z","creation":"2025-04-06T01:03:55.176Z"},"accession":"S-EPMC9913738","cross_references":{"pubmed":["36766770"],"doi":["10.3390/cells12030428"]}}