biostudies-literatureSojan JMEuropean community MSCA-ITN project BioMedAqu Horizon 2020364https://www.ebi.ac.uk/biostudies/studies/S-EPMC9913848biostudies-literatureUnknown12(3)Several in vivo trials have previously demonstrated the beneficial effects of the administration of various probiotic forms on bone health. In this study, we explored the potency of two probiotics, <i>Bacillus subtilis</i> and <i>Lactococcus lactis</i>, alone or in combination with vitamin D (VD), to modulate the transcription of genes involved in the ossification process in a human osteoblast cell line. Genes that mark the "osteoblast proliferation phase", such as <i>RUNX2</i>, <i>TGFB1</i>, and <i>ALPL</i>, "extracellular matrix (ECM) maturation", such as <i>SPP1</i> and <i>SPARC</i>, as well as "ECM mineralization", such as <i>BGN</i>, <i>BGLAP</i>, and <i>DCN</i>, were all highly expressed in osteoblasts treated with <i>B. subtilis</i> extract. The observed increase in the transcriptioCells<i>Bacillus subtilis</i> Modulated the Expression of Osteogenic Markers in a Human Osteoblast Cell Line.PMC9913848No 766347Licini CMarcheggiani FCarnevali OSojan JMMaradonna FTiano LMattioli-Belmonte Mfalse<i>Bacillus subtilis</i> Modulated the Expression of Osteogenic Markers in a Human Osteoblast Cell Line.Several in vivo trials have previously demonstrated the beneficial effects of the administration of various probiotic forms on bone health. In this study, we explored the potency of two probiotics, <i>Bacillus subtilis</i> and <i>Lactococcus lactis</i>, alone or in combination with vitamin D (VD), to modulate the transcription of genes involved in the ossification process in a human osteoblast cell line. Genes that mark the "osteoblast proliferation phase", such as <i>RUNX2</i>, <i>TGFB1</i>, and <i>ALPL</i>, "extracellular matrix (ECM) maturation", such as <i>SPP1</i> and <i>SPARC</i>, as well as "ECM mineralization", such as <i>BGN</i>, <i>BGLAP</i>, and <i>DCN</i>, were all highly expressed in osteoblasts treated with <i>B. subtilis</i> extract. The observed increase in the transcriptio2023-01-01T00:00:00Z2023 Jan2025-06-01T03:58:53.123Z2024-11-19T19:02:32.748ZS-EPMC99138483676670910.3390/cells12030364