biostudies-literatureJiao WIndustrial Innovation Team Program of Vegetables of Modern Agricultural Industry Technology System of Hebei ProvinceDouble First-Class Food Processing Discipline GroupHebei Province Key Research and Development Plan Project503https://www.ebi.ac.uk/biostudies/studies/S-EPMC9913893biostudies-literatureUnknown12(3)As the main bioactive component in dried ginger, 6-shogaol has potential hypoglycemic activity, but its mechanism is still unclear. The process of carbohydrate digestion and glucose absorption is closely related to the enzymatic activity of epithelial brush cells, expression of glucose transporters, and permeability of intestinal epithelial cells. Therefore, this study explored the hypoglycemic mechanism of 6-shogaol from the perspective of glucose uptake, absorption transport, and protection of intestinal barrier function. Based on molecular docking, the binding energy of 6-shogaol and α-glucosidase is -6.24 kcal/mol, showing a high binding affinity. Moreover, a-glucosidase enzymatic activity was reduced (-78.96%) when the 6-shogaol concentration was 500 µg/mL. After 6-shogaol interventioFoods (Basel, Switzerland)Effects of 6-Shogaol on Glucose Uptake and Intestinal Barrier Integrity in Caco-2 Cells.PMC9913893grant number 1090063-2grant number HBCT2018030208grant number 21327314DWang SWang XSang YJiao WfalseEffects of 6-Shogaol on Glucose Uptake and Intestinal Barrier Integrity in Caco-2 Cells.As the main bioactive component in dried ginger, 6-shogaol has potential hypoglycemic activity, but its mechanism is still unclear. The process of carbohydrate digestion and glucose absorption is closely related to the enzymatic activity of epithelial brush cells, expression of glucose transporters, and permeability of intestinal epithelial cells. Therefore, this study explored the hypoglycemic mechanism of 6-shogaol from the perspective of glucose uptake, absorption transport, and protection of intestinal barrier function. Based on molecular docking, the binding energy of 6-shogaol and α-glucosidase is -6.24 kcal/mol, showing a high binding affinity. Moreover, a-glucosidase enzymatic activity was reduced (-78.96%) when the 6-shogaol concentration was 500 µg/mL. After 6-shogaol interventio2023-01-01T00:00:00Z2023 Jan2025-04-03T22:57:09.2Z2025-02-19T03:26:05.494ZS-EPMC99138933676603210.3390/foods12030503