biostudies-literatureUnknownZhang PNIGMS NIH HHSApical constriction is a cell shape change that drives key morphogenetic events during development, including gastrulation and neural tube formation. The forces driving apical constriction are primarily generated through the contraction of apicolateral and/or medioapical actomyosin networks. In the <i>Drosophila</i> ventral furrow, the medioapical actomyosin network has a sarcomere-like architecture, with radially polarized actin filaments and centrally enriched non-muscle myosin II and myosin activating kinase. To determine if this is a broadly conserved actin architecture driving apical constriction, we examined actomyosin architecture during <i>C. elegans</i> gastrulation, in which two endodermal precursor cells internalize from the surface of the embryo. Quantification of protein localbioRxiv : the preprint server for biology2023.01.30.526280https://www.ebi.ac.uk/biostudies/studies/S-EPMC9915510biostudies-literatureArchitecture of the cortical actomyosin network driving apical constriction in <i>C. elegans</i>.PMC9915510R35 GM134838Medwig-Kinney TNGoldstein BZhang PfalseArchitecture of the cortical actomyosin network driving apical constriction in <i>C. elegans</i>.Apical constriction is a cell shape change that drives key morphogenetic events during development, including gastrulation and neural tube formation. The forces driving apical constriction are primarily generated through the contraction of apicolateral and/or medioapical actomyosin networks. In the <i>Drosophila</i> ventral furrow, the medioapical actomyosin network has a sarcomere-like architecture, with radially polarized actin filaments and centrally enriched non-muscle myosin II and myosin activating kinase. To determine if this is a broadly conserved actin architecture driving apical constriction, we examined actomyosin architecture during <i>C. elegans</i> gastrulation, in which two endodermal precursor cells internalize from the surface of the embryo. Quantification of protein local2023-01-01T00:00:00Z2023 Feb2025-08-15T03:05:45.635Z2025-04-05T18:36:05.419ZS-EPMC99155103677821810.1101/2023.01.30.526280