{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Matlik K"],"funding":["NINDS NIH HHS"],"pubmed_abstract":["Developing neurons undergo a progression of morphological and gene expression changes as they transition from neuronal progenitors to mature, multipolar neurons. Here we use RNA-seq and H3K4me3 and H3K27me3 ChIP-seq to analyze how chromatin modifications control gene expression in a specific type of CNS neuron, the mouse cerebellar granule cell (GC). We find that in proliferating GC progenitors (GCPs), H3K4me3/H3K27me3 bivalency is common at neuronal genes and undergoes dynamic changes that correlate with gene expression during migration and circuit formation. Expressing a fluorescent sensor for bivalent H3K4me3 and H3K27me3 domains revealed subnuclear bivalent foci in proliferating GCPs. Inhibiting H3K27 methyltransferases EZH1 and EZH2 <i>in vitro</i> and in organotypic cerebellar slices"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2023.02.02.526881"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9915618"],"repository":["biostudies-literature"],"pubmed_title":["Histone bivalency regulates the timing of cerebellar granule cell development."],"pmcid":["PMC9915618"],"funding_grant_id":["R21 NS114545"],"pubmed_authors":["Allis CD","Matlik K","Paul MR","Hatten ME","Govek EE"],"additional_accession":[]},"is_claimable":false,"name":"Histone bivalency regulates the timing of cerebellar granule cell development.","description":"Developing neurons undergo a progression of morphological and gene expression changes as they transition from neuronal progenitors to mature, multipolar neurons. Here we use RNA-seq and H3K4me3 and H3K27me3 ChIP-seq to analyze how chromatin modifications control gene expression in a specific type of CNS neuron, the mouse cerebellar granule cell (GC). We find that in proliferating GC progenitors (GCPs), H3K4me3/H3K27me3 bivalency is common at neuronal genes and undergoes dynamic changes that correlate with gene expression during migration and circuit formation. Expressing a fluorescent sensor for bivalent H3K4me3 and H3K27me3 domains revealed subnuclear bivalent foci in proliferating GCPs. Inhibiting H3K27 methyltransferases EZH1 and EZH2 <i>in vitro</i> and in organotypic cerebellar slices","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Feb","modification":"2026-04-08T14:22:17.152Z","creation":"2025-02-19T02:23:33.255Z"},"accession":"S-EPMC9915618","cross_references":{"pubmed":["36778390"],"doi":["10.1101/2023.02.02.526881"]}}