biostudies-literatureUnknownMatlik KNINDS NIH HHSDeveloping neurons undergo a progression of morphological and gene expression changes as they transition from neuronal progenitors to mature, multipolar neurons. Here we use RNA-seq and H3K4me3 and H3K27me3 ChIP-seq to analyze how chromatin modifications control gene expression in a specific type of CNS neuron, the mouse cerebellar granule cell (GC). We find that in proliferating GC progenitors (GCPs), H3K4me3/H3K27me3 bivalency is common at neuronal genes and undergoes dynamic changes that correlate with gene expression during migration and circuit formation. Expressing a fluorescent sensor for bivalent H3K4me3 and H3K27me3 domains revealed subnuclear bivalent foci in proliferating GCPs. Inhibiting H3K27 methyltransferases EZH1 and EZH2 <i>in vitro</i> and in organotypic cerebellar slicesbioRxiv : the preprint server for biology2023.02.02.526881https://www.ebi.ac.uk/biostudies/studies/S-EPMC9915618biostudies-literatureHistone bivalency regulates the timing of cerebellar granule cell development.PMC9915618R21 NS114545Allis CDMatlik KPaul MRHatten MEGovek EEfalseHistone bivalency regulates the timing of cerebellar granule cell development.Developing neurons undergo a progression of morphological and gene expression changes as they transition from neuronal progenitors to mature, multipolar neurons. Here we use RNA-seq and H3K4me3 and H3K27me3 ChIP-seq to analyze how chromatin modifications control gene expression in a specific type of CNS neuron, the mouse cerebellar granule cell (GC). We find that in proliferating GC progenitors (GCPs), H3K4me3/H3K27me3 bivalency is common at neuronal genes and undergoes dynamic changes that correlate with gene expression during migration and circuit formation. Expressing a fluorescent sensor for bivalent H3K4me3 and H3K27me3 domains revealed subnuclear bivalent foci in proliferating GCPs. Inhibiting H3K27 methyltransferases EZH1 and EZH2 <i>in vitro</i> and in organotypic cerebellar slices2023-01-01T00:00:00Z2023 Feb2026-04-08T14:22:17.152Z2025-02-19T02:23:33.255ZS-EPMC99156183677839010.1101/2023.02.02.526881