{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Henderson MI"],"funding":["National Eye Institute","NEI NIH HHS","National Heart, Lung, and Blood Institute","NHLBI NIH HHS"],"pagination":["4275-4285"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9916253"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["19(11)"],"pubmed_abstract":["Lipid nanoparticles containing messenger RNA (mRNA-LNPs) have launched to the forefront of nonviral delivery systems with their realized potential during the COVID-19 pandemic. Here, we investigate the impact of commonly used biological buffers on the performance and durability of mRNA-LNPs. We tested the compatibility of three common buffers─HEPES, Tris, and phosphate-buffered saline─with a DLin-MC3-DMA mRNA-LNP formulation before and after a single controlled freeze-thaw cycle. We hypothesized that buffer composition would affect lipid-aqueous phase separation. Indeed, the buffers imposed structural changes in LNP morphology as indicated by electron microscopy, differential scanning calorimetry, and membrane fluidity assays. We employed in vitro and in vivo models to measure mRNA transfe"],"journal":["Molecular pharmaceutics"],"pubmed_title":["Leveraging Biological Buffers for Efficient Messenger RNA Delivery via Lipid Nanoparticles."],"pmcid":["PMC9916253"],"funding_grant_id":["SAHAY 19XX0","R21EY031066-02","R01HL146736-01","R21 EY031066","R01 HL146736"],"pubmed_authors":["Eygeris Y","Sahay G","Herrera M","Henderson MI","Jozic A"],"additional_accession":[]},"is_claimable":false,"name":"Leveraging Biological Buffers for Efficient Messenger RNA Delivery via Lipid Nanoparticles.","description":"Lipid nanoparticles containing messenger RNA (mRNA-LNPs) have launched to the forefront of nonviral delivery systems with their realized potential during the COVID-19 pandemic. Here, we investigate the impact of commonly used biological buffers on the performance and durability of mRNA-LNPs. We tested the compatibility of three common buffers─HEPES, Tris, and phosphate-buffered saline─with a DLin-MC3-DMA mRNA-LNP formulation before and after a single controlled freeze-thaw cycle. We hypothesized that buffer composition would affect lipid-aqueous phase separation. Indeed, the buffers imposed structural changes in LNP morphology as indicated by electron microscopy, differential scanning calorimetry, and membrane fluidity assays. We employed in vitro and in vivo models to measure mRNA transfe","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Nov","modification":"2025-04-04T03:01:15.773Z","creation":"2025-04-04T03:01:15.773Z"},"accession":"S-EPMC9916253","cross_references":{"pubmed":["36129254"],"doi":["10.1021/acs.molpharmaceut.2c00587"]}}