biostudies-literatureAn DChina Postdoctoral Science FoundationNational Natural Science Foundation of China2147https://www.ebi.ac.uk/biostudies/studies/S-EPMC9916613biostudies-literatureUnknown24(3)Fluorescent chemosensors are powerful imaging tools used in a broad range of biomedical fields. However, the application of fluorescent dyes in bioimaging still remains challenging, with small Stokes shifts, interfering signals, background noise, and self-quenching on current microscope configurations. In this work, we reported a supramolecular cage (<b>C_A</b>) by coordination-driven self-assembly of benzothiadiazole derivatives and Eu(OTf)₃. The <b>C_A</b> exhibited high fluorescence with a quantum yield (QY) of 38.57%, good photoluminescence (PL) stability, and a large Stokes shift (153 nm). Furthermore, the CCK-8 assay against U87 glioblastoma cells verified the low cytotoxicity of <b>C_A</b>. We revealed that the designed probes could be used as U87 cells targeting bioimaging.International journal of molecular sciencesTailored Supramolecular Cage for Efficient Bio-Labeling.PMC99166132021M69290522101267, 21803059, U1904212, and U2004191Zhang HYShi LSong MPLi THao XQChen YAn DfalseTailored Supramolecular Cage for Efficient Bio-Labeling.Fluorescent chemosensors are powerful imaging tools used in a broad range of biomedical fields. However, the application of fluorescent dyes in bioimaging still remains challenging, with small Stokes shifts, interfering signals, background noise, and self-quenching on current microscope configurations. In this work, we reported a supramolecular cage (<b>C_A</b>) by coordination-driven self-assembly of benzothiadiazole derivatives and Eu(OTf)₃. The <b>C_A</b> exhibited high fluorescence with a quantum yield (QY) of 38.57%, good photoluminescence (PL) stability, and a large Stokes shift (153 nm). Furthermore, the CCK-8 assay against U87 glioblastoma cells verified the low cytotoxicity of <b>C_A</b>. We revealed that the designed probes could be used as U87 cells targeting bioimaging.2023-01-01T00:00:00Z2023 Jan2025-04-25T19:21:33.393Z2025-04-06T07:58:39.323ZS-EPMC99166133676847110.3390/ijms24032147