biostudies-literatureAravindraja CNIH HHS2327https://www.ebi.ac.uk/biostudies/studies/S-EPMC9916963biostudies-literatureUnknown24(3)<i>Porphyromonas gingivalis</i> is one of the major bacteria constituting the subgingival pathogenic polymicrobial milieu during periodontitis. Our objective is to determine the global microRNA (miRNA, miR) expression kinetics in 8- and 16-weeks duration of <i>P. gingivalis</i> infection in C57BL/6J mice and to identify the miRNA signatures at specific time-points in mice. We evaluated differential expression (DE) miRNAs in mandibles (<i>n</i> = 10) using high-throughput NanoString nCounter^® miRNA expression panels. The bacterial colonization, alveolar bone resorption (ABR), serum immunoglobulin G (IgG) antibodies, and bacterial dissemination were confirmed. In addition, all the infected mice showed bacterial colonization on the gingival surface, significant increases in ABR (<iInternational journal of molecular sciencesSpecific microRNA Signature Kinetics in <i>Porphyromonas gingivalis</i>-Induced Periodontitis.PMC9916963R01 DE028536Botello-Escalante RChan EKLKesavalu LAravindraja CVekariya KMRahaman SOfalseSpecific microRNA Signature Kinetics in <i>Porphyromonas gingivalis</i>-Induced Periodontitis.<i>Porphyromonas gingivalis</i> is one of the major bacteria constituting the subgingival pathogenic polymicrobial milieu during periodontitis. Our objective is to determine the global microRNA (miRNA, miR) expression kinetics in 8- and 16-weeks duration of <i>P. gingivalis</i> infection in C57BL/6J mice and to identify the miRNA signatures at specific time-points in mice. We evaluated differential expression (DE) miRNAs in mandibles (<i>n</i> = 10) using high-throughput NanoString nCounter^® miRNA expression panels. The bacterial colonization, alveolar bone resorption (ABR), serum immunoglobulin G (IgG) antibodies, and bacterial dissemination were confirmed. In addition, all the infected mice showed bacterial colonization on the gingival surface, significant increases in ABR (<i2023-01-01T00:00:00Z2023 Jan2025-04-25T19:18:15.048Z2025-04-06T07:57:11.238ZS-EPMC99169633676865110.3390/ijms24032327