biostudies-literatureKayashima YNICHD NIH HHSNIH HHS2149https://www.ebi.ac.uk/biostudies/studies/S-EPMC9917068biostudies-literatureUnknown24(3)Obesity is a risk factor for preeclampsia. We investigated how obesity influences preeclampsia in mice lacking ankyrin-repeat-and-SOCS-box-containing-protein 4 (ASB4), which promotes trophoblast differentiation via degrading the inhibitor of DNA-binding protein 2 (ID2). <i>Asb4^-/-</i> mice on normal chow (NC) develop mild preeclampsia-like phenotypes during pregnancy, including hypertension, proteinuria, and reduced litter size. Wild-type (WT) and <i>Asb4^-/-</i> females were placed on a high-fat diet (HFD) starting at weaning. At the age of 8-9 weeks, they were mated with WT or <i>Asb4^-/-</i> males, and preeclamptic phenotypes were assessed. HFD-WT dams had no obvious adverse outcomes of pregnancy. In contrast, HFD-<i>Asb4</i>^-/- dams had signifiInternational journal of molecular sciencesInsulin Elevates ID2 Expression in Trophoblasts and Aggravates Preeclampsia in Obese ASB4-Null Mice.PMC9917068R01 HD101485R01HL049277, R01HD101485, P30 DK 034987Vora NLBoggess KMaeda-Smithies NHomeister JWLi FTownley-Tilson WHDKayashima YfalseInsulin Elevates ID2 Expression in Trophoblasts and Aggravates Preeclampsia in Obese ASB4-Null Mice.Obesity is a risk factor for preeclampsia. We investigated how obesity influences preeclampsia in mice lacking ankyrin-repeat-and-SOCS-box-containing-protein 4 (ASB4), which promotes trophoblast differentiation via degrading the inhibitor of DNA-binding protein 2 (ID2). <i>Asb4^-/-</i> mice on normal chow (NC) develop mild preeclampsia-like phenotypes during pregnancy, including hypertension, proteinuria, and reduced litter size. Wild-type (WT) and <i>Asb4^-/-</i> females were placed on a high-fat diet (HFD) starting at weaning. At the age of 8-9 weeks, they were mated with WT or <i>Asb4^-/-</i> males, and preeclamptic phenotypes were assessed. HFD-WT dams had no obvious adverse outcomes of pregnancy. In contrast, HFD-<i>Asb4</i>^-/- dams had signifi2023-01-01T00:00:00Z2023 Jan2025-05-29T22:23:22.89Z2024-11-09T13:03:52.779ZS-EPMC99170683676846910.3390/ijms24032149