{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Murata T"],"funding":["Takeda Science Foundation","Japan Agency for Medical Research and Development"],"pagination":["2265"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9917364"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["24(3)"],"pubmed_abstract":["N6-methyladenosine (m<sup>6</sup>A) is a post-transcriptional modification of RNA involved in transcript transport, degradation, translation, and splicing. We found that HBV RNA is modified by m<sup>6</sup>A predominantly in the coding region of <i>HBx</i>. The mutagenesis of methylation sites reduced the HBV mRNA and HBs protein levels. The suppression of m<sup>6</sup>A by an inhibitor or knockdown in primary hepatocytes decreased the viral RNA and HBs protein levels in the medium. These results suggest that the m<sup>6</sup>A modification of HBV RNA is needed for the efficient replication of HBV in hepatocytes."],"journal":["International journal of molecular sciences"],"pubmed_title":["N6-methyladenosine Modification of Hepatitis B Virus RNA in the Coding Region of <i>HBx</i>."],"pmcid":["PMC9917364"],"funding_grant_id":["na","JP20fk0310104, 22fk0310507"],"pubmed_authors":["Murata T","Iwahori S","Kimura H","Nishitsuji H","Shimotohno K","Okuno Y","Yanagi Y","Watashi K","Wakita T"],"additional_accession":[]},"is_claimable":false,"name":"N6-methyladenosine Modification of Hepatitis B Virus RNA in the Coding Region of <i>HBx</i>.","description":"N6-methyladenosine (m<sup>6</sup>A) is a post-transcriptional modification of RNA involved in transcript transport, degradation, translation, and splicing. We found that HBV RNA is modified by m<sup>6</sup>A predominantly in the coding region of <i>HBx</i>. The mutagenesis of methylation sites reduced the HBV mRNA and HBs protein levels. The suppression of m<sup>6</sup>A by an inhibitor or knockdown in primary hepatocytes decreased the viral RNA and HBs protein levels in the medium. These results suggest that the m<sup>6</sup>A modification of HBV RNA is needed for the efficient replication of HBV in hepatocytes.","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jan","modification":"2025-04-19T17:18:48.613Z","creation":"2025-02-19T03:25:54.2Z"},"accession":"S-EPMC9917364","cross_references":{"pubmed":["36768585"],"doi":["10.3390/ijms24032265"]}}