{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["14(1)"],"submitter":["Tee YH"],"pubmed_abstract":["Deviations from mirror symmetry in the development of bilateral organisms are common but the mechanisms of initial symmetry breaking are insufficiently understood. The actin cytoskeleton of individual cells self-organises in a chiral manner, but the molecular players involved remain essentially unidentified and the relationship between chirality of an individual cell and cell collectives is unclear. Here, we analysed self-organisation of the chiral actin cytoskeleton in individual cells on circular or elliptical patterns, and collective cell alignment in confined microcultures. Screening based on deep-learning analysis of actin patterns identified actin polymerisation regulators, depletion of which suppresses chirality (mDia1) or reverses chirality direction (profilin1 and CapZβ). The reve"],"journal":["Nature communications"],"pagination":["776"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9922260"],"repository":["biostudies-literature"],"pubmed_title":["Actin polymerisation and crosslinking drive left-right asymmetry in single cell and cell collectives."],"pmcid":["PMC9922260"],"pubmed_authors":["Jalal S","Barnett SFH","Yan J","Shi S","Goh WJ","Mogilner A","Yong X","Ong HT","Thiagarajan V","Tee YH","Bershadsky AD","Hu J","Huang W","Lim YAB","Tay IYY","Kanchanawong P"],"additional_accession":[]},"is_claimable":false,"name":"Actin polymerisation and crosslinking drive left-right asymmetry in single cell and cell collectives.","description":"Deviations from mirror symmetry in the development of bilateral organisms are common but the mechanisms of initial symmetry breaking are insufficiently understood. The actin cytoskeleton of individual cells self-organises in a chiral manner, but the molecular players involved remain essentially unidentified and the relationship between chirality of an individual cell and cell collectives is unclear. Here, we analysed self-organisation of the chiral actin cytoskeleton in individual cells on circular or elliptical patterns, and collective cell alignment in confined microcultures. Screening based on deep-learning analysis of actin patterns identified actin polymerisation regulators, depletion of which suppresses chirality (mDia1) or reverses chirality direction (profilin1 and CapZβ). The reve","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Feb","modification":"2026-05-28T11:26:07.358Z","creation":"2025-02-19T03:26:08.937Z"},"accession":"S-EPMC9922260","cross_references":{"pubmed":["36774346"],"doi":["10.1038/s41467-023-35918-1"]}}