biostudies-literaturePillai MMIndian Institute of Technology BombayDepartment of Biotechnology, Ministry of Science and Technology, India102025https://www.ebi.ac.uk/biostudies/studies/S-EPMC9922965biostudies-literatureUnknown10Microneedle patches have been widely used in a minimally invasive manner for various drug delivery applications. However, for developing these microneedle patches, master molds are required, which are generally made of metal and are very expensive. Two-photon polymerization (2PP) technique can be used for fabricating microneedles more precisely and at a much lower cost. This study reports a novel strategy for developing microneedle master templates using the 2PP method. The main advantage of this technique is that there is no requirement for post-processing after laser writing, and that for the fabrication of polydimethylsiloxane (PDMS) molds, harsh chemical treatments such as silanization are not required. This is a one-step process for manufacturing of microneedle templates which allows MethodsXTwo-photon polymerization based reusable master template to fabricate polymer microneedles for drug delivery.PMC9922965BT/INF/22/SP23026/2017Pillai MMTayalia PAjesh SfalseTwo-photon polymerization based reusable master template to fabricate polymer microneedles for drug delivery.Microneedle patches have been widely used in a minimally invasive manner for various drug delivery applications. However, for developing these microneedle patches, master molds are required, which are generally made of metal and are very expensive. Two-photon polymerization (2PP) technique can be used for fabricating microneedles more precisely and at a much lower cost. This study reports a novel strategy for developing microneedle master templates using the 2PP method. The main advantage of this technique is that there is no requirement for post-processing after laser writing, and that for the fabrication of polydimethylsiloxane (PDMS) molds, harsh chemical treatments such as silanization are not required. This is a one-step process for manufacturing of microneedle templates which allows 2023-01-01T00:00:00Z20232025-04-20T01:35:01.724Z2025-04-20T01:35:01.724ZS-EPMC99229653679367410.1016/j.mex.2023.102025