{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["6(1)"],"submitter":["Schueth A"],"pubmed_abstract":["The ability to image human tissue samples in 3D, with both cellular resolution and a large field of view (FOV), can improve fundamental and clinical investigations. Here, we demonstrate the feasibility of light-sheet imaging of ~5 cm<sup>3</sup> sized formalin fixed human brain and up to ~7 cm<sup>3</sup> sized formalin fixed paraffin embedded (FFPE) prostate cancer samples, processed with the FFPE-MASH protocol. We present a light-sheet microscopy prototype, the cleared-tissue dual view Selective Plane Illumination Microscope (ct-dSPIM), capable of fast 3D high-resolution acquisitions of cm<sup>3</sup> scale cleared tissue. We used mosaic scans for fast 3D overviews of entire tissue samples or higher resolution overviews of large ROIs with various speeds: (a) Mosaic 16 (16.4 µm isotropic resolution, ~1.7 h/cm<sup>3</sup>), (b) Mosaic 4 (4.1 µm isotropic resolution, ~ 5 h/cm<sup>3</sup>) and (c) Mosaic 0.5 (0.5 µm near isotropic resolution, ~15.8 h/cm<sup>3</sup>). We could visualise cortical layers and neurons around the border of human brain areas V1&V2, and could demonstrate suitable imaging quality for Gleason score grading in thick prostate cancer samples. We show that ct-dSPIM imaging is an excellent technique to quantitatively assess entire MASH prepared large-scale human tissue samples in 3D, with considerable future clinical potential."],"journal":["Communications biology"],"pagination":["170"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9925784"],"repository":["biostudies-literature"],"pubmed_title":["Efficient 3D light-sheet imaging of very large-scale optically cleared human brain and prostate tissue samples."],"pmcid":["PMC9925784"],"pubmed_authors":["Zur Hausen A","Schueth A","Kiessling A","Hildebrand S","Samarska I","Herrler A","Sengupta S","Capalbo M","Roebroeck A"],"additional_accession":[]},"is_claimable":false,"name":"Efficient 3D light-sheet imaging of very large-scale optically cleared human brain and prostate tissue samples.","description":"The ability to image human tissue samples in 3D, with both cellular resolution and a large field of view (FOV), can improve fundamental and clinical investigations. Here, we demonstrate the feasibility of light-sheet imaging of ~5 cm<sup>3</sup> sized formalin fixed human brain and up to ~7 cm<sup>3</sup> sized formalin fixed paraffin embedded (FFPE) prostate cancer samples, processed with the FFPE-MASH protocol. We present a light-sheet microscopy prototype, the cleared-tissue dual view Selective Plane Illumination Microscope (ct-dSPIM), capable of fast 3D high-resolution acquisitions of cm<sup>3</sup> scale cleared tissue. We used mosaic scans for fast 3D overviews of entire tissue samples or higher resolution overviews of large ROIs with various speeds: (a) Mosaic 16 (16.4 µm isotropic resolution, ~1.7 h/cm<sup>3</sup>), (b) Mosaic 4 (4.1 µm isotropic resolution, ~ 5 h/cm<sup>3</sup>) and (c) Mosaic 0.5 (0.5 µm near isotropic resolution, ~15.8 h/cm<sup>3</sup>). We could visualise cortical layers and neurons around the border of human brain areas V1&V2, and could demonstrate suitable imaging quality for Gleason score grading in thick prostate cancer samples. We show that ct-dSPIM imaging is an excellent technique to quantitatively assess entire MASH prepared large-scale human tissue samples in 3D, with considerable future clinical potential.","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Feb","modification":"2025-04-18T21:32:11.219Z","creation":"2025-04-07T09:24:45.677Z"},"accession":"S-EPMC9925784","cross_references":{"pubmed":["36781939"],"doi":["10.1038/s42003-023-04536-4"]}}