{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Chuapoco MR"],"funding":["NIMH NIH HHS","NIH HHS"],"pubmed_abstract":["Adeno-associated viruses (AAVs) promise robust gene delivery to the brain through non-invasive, intravenous delivery. However, unlike in rodents, few neurotropic AAVs efficiently cross the blood-brain barrier in non-human primates (NHPs). Here we describe AAV.CAP-Mac, an engineered variant identified by screening in adult marmosets and newborn macaques with improved efficiency in the brain of multiple NHP species: marmoset, rhesus macaque, and green monkey. CAP-Mac is neuron-biased in infant Old World primates, exhibits broad tropism in adult rhesus macaques, and is vasculature-biased in adult marmosets. We demonstrate applications of a single, intravenous dose of CAP-Mac to deliver (1) functional GCaMP for ex vivo calcium imaging across multiple brain areas, and (2) a cocktail of fluoresc"],"journal":["Research square"],"pagination":["rs.3.rs-1370972"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9928057"],"repository":["biostudies-literature"],"pubmed_title":["Intravenous functional gene transfer throughout the brain of non-human primates using AAV."],"pmcid":["PMC9928057"],"funding_grant_id":["UF1 MH128336","P51 OD011107"],"pubmed_authors":["Deverman BE","Lefevre A","Fox AS","Man KNM","Taskin N","Octeau JC","Campos LJ","Bishaw Y","Winchester J","Weed N","Scherrer J","Mich J","Vendemiatti J","Gradinaru V","Arokiaraj CM","Blackburn RJ","Chuapoco MR","Chen X","Shaya TF","Pickel J","Chan KY","Sun J","Flytzanis NC","Singh VP","Tian L","Gore B","Omstead V","Miller CT","Ting J","Goeden N","Roxas KM","Jang MJ"],"additional_accession":[]},"is_claimable":false,"name":"Intravenous functional gene transfer throughout the brain of non-human primates using AAV.","description":"Adeno-associated viruses (AAVs) promise robust gene delivery to the brain through non-invasive, intravenous delivery. However, unlike in rodents, few neurotropic AAVs efficiently cross the blood-brain barrier in non-human primates (NHPs). Here we describe AAV.CAP-Mac, an engineered variant identified by screening in adult marmosets and newborn macaques with improved efficiency in the brain of multiple NHP species: marmoset, rhesus macaque, and green monkey. CAP-Mac is neuron-biased in infant Old World primates, exhibits broad tropism in adult rhesus macaques, and is vasculature-biased in adult marmosets. We demonstrate applications of a single, intravenous dose of CAP-Mac to deliver (1) functional GCaMP for ex vivo calcium imaging across multiple brain areas, and (2) a cocktail of fluoresc","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jan","modification":"2026-06-04T03:10:01.193Z","creation":"2025-04-04T13:07:29.351Z"},"accession":"S-EPMC9928057","cross_references":{"pubmed":["36789432"],"doi":["10.21203/rs.3.rs-1370972/v1"]}}